核移植技术在建立乳腺生物反应器中的应用

乳腺生物反应器的开发为制药业带来了生机,但目前采用的动物基因转移的方法,由于其各自固有的局限性,未能使得乳腺生物反应器的研究取得长足的进步。而核移植技术的发展为乳腺生物反应器的开发注入了活力。因此,笔者综合近年来国内外的文献,提出了核移植中供体细胞的选择以及处理方法,同时也从分子水平阐述了乳腺的一些调控机制,为核移植技术在乳腺生物反应器中的应用奠定了基础。

Application of production of mammary gland bioreactor by nuclear transfer

The development of mammary gland bioreactor brings vitality for the pharmaceutical industry. but, the methods of transgene transfer, because of their inherent limitation, so, it didn't make a further progress in the mammary gland bioreactor, while, the nuclear transfer brings the vitality for the development of mammary gland bioreactor. So,integrated the domestic and foreign documents and provided the methods of selection and treatment of donor cell in nuclear transfer. At the same time, detailed the regulate mechanism of the mammary gland in the molecular level, which laid the foundation of nuclear transfer.