| 剪切力对链霉菌谷氨酰胺转胺酶发酵的影响 |
| |
| 引用本文： | 范西强,陈艳娟,宋敏,张颖颖,常忠义,高红亮.剪切力对链霉菌谷氨酰胺转胺酶发酵的影响[J].华北农学报,2008,23(Z2). |
| |
| 作者姓名： | 范西强 陈艳娟 宋敏 张颖颖 常忠义 高红亮 |
| |
| 作者单位： | 华东师范大学,生命科学学院,上海,200062 |
| |
| 基金项目： | 青年自然科学基金项目(30600079) |
| |
| 摘 要： | 考察了以摇瓶发酵过程中添加玻珠为剪切力,对链霉菌(Streptomyces sp.)HS-1发酵生产谷氨酰胺转胺酶的影响,在10 L发酵罐上不同搅拌转速对产酶的影响。结果显示,摇瓶发酵中,在种子阶段添加玻珠,提高剪切力不能促进产酶;在发酵阶段,加入4颗玻珠时,酶活相对最高,比对照增加20%;36 h时添加玻珠,与对照相比,酶活提高12%,菌体浓度增加40%,且氨基氮消耗明显。10 L发酵罐中,适宜的搅拌转速为250 r/min,36 h调整转速至250r/min,酶活比维持90 r/min提高21%。因此剪切力对谷氨酰胺转胺酶发酵有明显影响。
|
| 关 键 词： | 链霉菌 剪切力 谷氨酰胺转胺酶 玻珠 |
Effects of Shear Force on Microbial Transglutaminase Fermentation |
| |
| FAN Xi-qiang,CHEN Yan-juan,SONG Min,ZHANG Ying-ying,CHANG Zhong-yi,GAO Hong-liang.Effects of Shear Force on Microbial Transglutaminase Fermentation[J].Acta Agriculturae Boreali-Sinica,2008,23(Z2). |
| |
| Authors: | FAN Xi-qiang CHEN Yan-juan SONG Min ZHANG Ying-ying CHANG Zhong-yi GAO Hong-liang |
| |
| Abstract: | The influences of glass beads on microbial transglutaminase fermentation in flask were studied.Effects of shear force on microbial transglutaminase fermentation were also studied in a 10 L fermenter by adjusting the agitation speed.The results showed that enhancing agitation speed has no significant effect on MTG activity at the duration of inoculum.MTG activity increased by 20% when four glass beads were put in the flask.Increasing shear force at 36 h would promote MTG synthesis.MTG activity and cell conce... |
| |
| Keywords: | Streptomyces sp Shear force Transglutaminase Glass bead |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
