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| 利用SSR标记快速鉴定玉米杂交种农大108和豫玉27的种子纯度 | |
| 引用本文： | 高文伟,李晓辉,田清震,李明顺,李新海,张世煌. 利用SSR标记快速鉴定玉米杂交种农大108和豫玉27的种子纯度[J]. 种子, 2004, 23(5): 32-33 |
| 作者姓名： | 高文伟 李晓辉 田清震 李明顺 李新海 张世煌 |
| 作者单位： | 1. 新疆农业大学农学系,乌鲁木齐,830052 2. 东北农业大学农学院,哈尔滨,150030 3. 中国农业科学院作物育种栽培研究所,农业部作物遗传育种重点开放实验室,北京,100081 |
| 摘 要： | 本文利用SSR标记技术分别筛选出玉米杂交种农大108和豫玉27的特异性引物,结合单籽粒DNA快速提取方法,建立了两个品种的种子纯度鉴定试剂盒.实验表明,应用此试剂盒可快速、有效地鉴定两个杂交种的种子纯度. |
| 关 键 词： | 玉米 杂交种 SSR标记 种子纯度鉴定 |
Seed Purity Testing of Maize Hybrid Nongda108 and Yu27 by SSR Marker Analysis |
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| Gao Wenwei,Li Xiaohui,Tian Qingzhen,Li Mingshun,Li Xinhai,Zhang Shihuang. Seed Purity Testing of Maize Hybrid Nongda108 and Yu27 by SSR Marker Analysis[J]. Seed, 2004, 23(5): 32-33 | |
| Authors: | Gao Wenwei Li Xiaohui Tian Qingzhen Li Mingshun Li Xinhai Zhang Shihuang |
| Affiliation: | Gao Wenwei1,Li Xiaohui2,Tian Qingzhen3,Li Mingshun3,Li Xinhai3,Zhang Shihuang3 |
| Abstract: | Polymorphic SSR primers were screened between the parental lines of maize hybrids Nongda108 and Yuyu27, respectively. With the adoption of DNA rapid extraction method and SSR marker system, the seed purity testing kits of two hybrids were established. The analysis showed that the testing kits could be effectively and rapidly used for seed purity analysis of two hybrids. |
| Keywords: | Zea mays L. Hybrid SSR marker Seed purity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |