两株抗铜细菌的筛选、鉴定及对碳酸铜的溶解作用

本研究从长期受铜污染的铜矿废弃地土壤中分离得到两株对重金属铜具有较强抗性的菌株F16a和Fw17a。对菌株形态、生理生化特性及其16S rDNA系统进化进行分析,将F16a鉴定为肠杆菌属(Enterobacter),Fw17a鉴定为假单胞菌属(Pseudomona)。F16a和Fw17a对氨苄青霉素、头孢他啶均有抗性;此外,Fw17a还对氯霉素、四环素及低浓度的卡那霉素具有抗性。在有氮培养基(含500 mg/L CuCO3)液体培养48 h后,F16a使培养基上清液中铜浓度增加了300%左右;相反,Fw17a使培养基上清液中铜浓度降低了60%左右。

Isolation and Biological Characterization of Two Copper-resistant Bacteria and Their Solubilization of Copper Carbonate

In this study, Cu-resistant bacteria strains F16a and Fw17a were isolated from Cu-contaminated soil in copper mining wasteland, the strains F16a and Fw17a were identified preliminarily as Enterobacter sp. and Pseudomona sp., respectively, based on their morphological, physiological and biochemical characteristics and 16S rDNA sequences. The strains F16a and Fw17a exhibited the resistance to ampicillin and ceftazidime, and moreover, Fw17a was also resistant to chloramphenicol, tetracyclin and lower concentration kanamycin. F16a significantly increased the concentration of water-soluble copper in supernatant of liquid N-containing medium with 500 mg/L CuCO3, the concentration of water-soluble copper in supernatant was increased by 300% of water-soluble copper concentration in control. However, Fw17a decreased the concentration of water-soluble copper in supernatant by close to 60% compared with the control under the same conditions.