镉对中间球海胆精子发生的影响

研究了不同浓度镉离子(0.005、0.1、0.5、1.0 mg/L)对中间球海胆Strongylocentrotus interm edius精子发生的影响。结果显示:在光镜下观察,染毒第6、18 d后,各浓度组中间球海胆滤泡结构完好,生殖细胞发育情况同对照组基本一样;染毒第42 d后,0.5、1.0 mg/L Cd2 浓度组生精细胞排列混乱,滤泡膜破损;染毒第54 d后,各浓度组海胆性腺均出现滤泡解体现象。对染Cd2 54 d的0.5、1.0 mg/L组海胆精巢进行超薄切片观察,可见各级生精细胞亚显微结构变化明显,主要表现为核染色质异常凝集,核膜结构损伤,线粒体空泡化,精子形态畸形等。说明镉能影响中间球海胆的精子发生,导致精子质量下降。

The effects of cadmium on spermatogenesis in sea urchin Strongylocentrotus intermedius

The effects of cadmium(0.005,0.1,0.5,and 1.0 mg/L)on spermatogenesis in sea urchin Strongylocentrotus intermedius were studied.There was no significant alteration in the gonadal acini after 6 and 18 d of Cd2 exposure.The spermatogenic cells were the same as those in the control.After 42 d Cd2 exposure,disorder arrangement of spermatogenic cells and damaged membranes of gonadal acini were observed in the Cd2 0.5 mg/L and 1.0 mg/L groups.Disorganization occurred at the end of the experiment.Spermatogenic cells had ultrastructural changes after 54 d cadmium exposure in the Cd2 0.5 mg/L and 1.0 mg/L groups including the nucleus condensed abnormally,disintegrated membrane structure,and vacuolated mitochondria.The alterations led to the malformation of sperm,indicating that cadmium ions affect the spermatogenesis and sperm quality in the sea urchin.