| 南京地区犬细小病毒基因型鉴定 |
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| 引用本文： | 方光远,樊金云,谈福利,金海燕,黄心昱,王喜国,张玉红,陈俊红,王楠楠,戴鼎震. 南京地区犬细小病毒基因型鉴定[J]. 畜牧与兽医, 2021, 0(3): 95-101 |
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| 作者姓名： | 方光远 樊金云 谈福利 金海燕 黄心昱 王喜国 张玉红 陈俊红 王楠楠 戴鼎震 |
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| 作者单位： | 金陵科技学院动物科学与技术学院;乾元浩生物股份有限公司南京生物药厂 |
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| 基金项目： | 金陵科技学院2019年校级大学生创新训练计划项目(201913573019Z);金陵科技学院2020年校级大学生创新训练计划项目(202013573017Z);金陵科技学院2020年度“创客”虚拟班项目(2017008)。 |
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| 摘 要： | 为调查南京地区犬细小病毒(canine parvovirus,CPV)基因型分布情况,采集南京市某宠物医院2012-2019年犬细小病毒病患犬粪便43份作为检测病料,通过PCR扩增犬细小病毒VP2基因片段,并将扩增产物测序,分析CPV基因型.结果显示,43份病料皆扩增出目的片段,经测序后使用MegAlign软件与Gen...
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| 关 键 词： | 犬细小病毒 基因型 鉴定 南京 |
Genotype identification of canine parvovirus in the Nanjing area |
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| FANG Guangyuan,FAN Jinyun,TAN Fuli,JIN Haiyan,HUANG Xinyu,WANG Xiguo,ZHANG Yuhong,CHEN Junhong,WANG Nannan,DAI Dingzhen. Genotype identification of canine parvovirus in the Nanjing area[J]. Animal Husbandry & Veterinary Medicine, 2021, 0(3): 95-101 |
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| Authors: | FANG Guangyuan FAN Jinyun TAN Fuli JIN Haiyan HUANG Xinyu WANG Xiguo ZHANG Yuhong CHEN Junhong WANG Nannan DAI Dingzhen |
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| Affiliation: | (College of Animal Science and Technology,Jinling Institute of Technology,Nanjing 210038,China;Qianyuanhao Biological Co.Ltd.Nanjing Biological Pharmaceutical Factory,Nanjing 210012,China) |
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| Abstract: | In order to investigate the genotype distribution of canine parvovirus(CPV)in the Nanjing area,43 feces samples of dogs infected with canine parvovirus in a pet hospital in Nanjing in the period from 2012 to 2019 were collected as test materials.The VP2 Gene fragments of the canine parvovirus were amplified by PCR,and the amplified products were sequenced to analyze its genotype.The results showed that target fragments were amplified from all the 43 diseased materials.After sequencing,the MegAlign software was used to compare the fragments with the known genotypes of canine parvovirus in GenBank.It was found that 26 fragments of the strain were of the New-CPV-2 a genotype,accounting for 60.5%of the total;that nine were of the New-CPV-2 b genotype,accounting for 20.9%;and that eight were of the CPV-2 c genotype,accounting for 18.6%.The present study indicated that the genotype of canine parvovirus in the Nanjing area had undergone a wide variation in recent years,that New-CPV-2 a was the dominant genotype,and that both New-CPV-2 b and CPV-2 c genotypes existed simultaneously.These results provided a theoretical basis for prevention and treatment of canine parvovirus diseases in the Nanjing area. |
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| Keywords: | canine parvovirus genotype identification Nanjing |
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