牛瘤胃上皮细胞中挥发性脂肪酸吸收相关蛋白在白细胞介素10诱导下的差异表达研究

本研究旨在探讨炎症因子(白细胞介素10,IL-10)对牛瘤胃上皮细胞中挥发性脂肪酸(VFA)吸收相关蛋白基因表达的影响.试验采用随机区组设计,在瘤胃上皮细胞体外培养条件下,研究50 ng·mL-1浓度的IL-10对牛瘤胃上皮细胞中核因子(NF-κB)、假定阴离子转运蛋白1(PAT1)、阴离子交换蛋白2(AE2)、单羧酸...

Differential Expression of Volatile Fatty Acid Absorption Related Proteins in Bovine Rumen Epithelial Cells Induced by Interleukin 10

This study aimed to investigate the effect of inflammatory factor(interleukin-10,IL-10)on the expression of genes related to volatile fatty acid(VFA)absorption in bovine rumen epithelial cells.The experiment adopted a randomized block design.Under the conditions of in vitro culture of rumen epithelial cells,the effect of 50 ng·mL^-1 IL-10 on genes expression of nuclear factor(NF-κB),putative anion transporter 1(PAT 1),anion exchanger 2(AE 2),monocarboxylate transporter 1(MCT 1),monocarboxylate transporter 4(MCT 4),Na-/H+exchanger 1(NHE 1)in bovine rumen epithelial cells was detected.The results showed that,under neutral conditions,IL-10 addition significantly down-regulated the expression of NF-κB,PAT 1,MCT 1,NHE 1 genes(P<0.05),and significantly up-regulated the expression of AE 2 gene(P<0.05),while had no significant effect on the gene expression of MC T4.Under acidic conditions,the expression of NF-κB gene in the IL-10 addition group was significantly inhibited(P<0.05),while the expression of PAT 1,MCT 1,and MCT 4 genes were significantly promoted(P<0.05).Compared with the condition of pH7.2 group,the NF-κB gene expression in the pH5.5 group was significantly increased(P<0.05).The results suggest that,under inflammatory conditions,interleukin-10 as an anti-inflammatory factor can alleviate the inflammatory response of rumen epithelial cells and promote the genes expression of VFA absorption-related protein.