白皮杉醇对氧化应激断奶仔猪空肠抗氧化能力、黏膜形态和屏障功能的影响

旨在探究灌喂白皮杉醇对敌草快刺激断奶仔猪空肠氧化损伤、黏膜形态及屏障功能的影响。本研究选取健康状况良好的28日龄“杜×长×大”三元杂交雄性断奶仔猪54头，随机分为3组（每组6个重复，每个重复3头猪），平均体重为（8.13±0.41）kg。对照组（CON）和敌草快组（DQ-CON）每天灌喂0.5%羧甲基纤维素钠溶液，敌草快+白皮杉醇组（DQ-PIC）灌喂含有白皮杉醇（80 mg·kg^-1）的0.5%羧甲基纤维素钠溶液。在屠宰前24 h，DQ-CON组和DQ-PIC组分别注射10 mg·kg^-1敌草快溶液，CON组注射等量生理盐水，试验期共计8 d。36日龄屠宰后测定各组仔猪空肠肠道损伤情况以及抗氧化酶活和基因表达情况。结果表明：1）与CON组相比，DQ-CON组仔猪血浆二胺氧化酶（DAO）含量显著升高（P<0.05），空肠紧密连接蛋白（OCLN）基因表达量显著下降（P<0.05）；与DQ-CON组相比，DQ-PIC组仔猪血浆DAO含量显著下降（P<0.05），OCLN基因表达量显著上升（P<0.05）。2）与CON组相比，DQ-CON组仔猪空肠黏膜总超氧化物歧化酶（T-SOD）活性、过氧化氢酶（CAT）活性、超氧化物歧化酶1（SOD1）基因相对表达量显著下降（P<0.05），丙二醛（MDA）含量显著上升（P<0.05）；与DQ-CON组相比，DQ-PIC组仔猪空肠黏膜T-SOD活性、CAT活性、SOD1基因相对表达量、HO1基因相对表达量显著上升（P<0.05），MDA含量显著下降（P<0.05）。由此可知，灌喂白皮杉醇能够缓解敌草快诱导的断奶仔猪空肠氧化应激损伤，提高空肠抗氧化能力，促进空肠黏膜形态和肠道屏障功能的恢复。

Effect of Piceatannol on the Antioxidant Capacity,Mucosal Morphology and Barrier Function of the Jejunum of Weaned Piglets under Oxidative Stress

This study aimed to explore the effect of piceatannol (PIC) on the oxidative damage, mucosal morphology, and barrier function in the jejunum of weaned piglets challenged with diquat (DQ). Fifty-four male piglets (Duroc×Landrace×Yorkshire]) in good health with an average body weight (BW) of (8.13±0.41)kg were selected and randomly divided into 3 groups (each treatment group contained 6 replicates with 3 piglets per replicate). Piglets in the CON and DQ-CON groups were orally administered with a vehicle solution contained 0.5% carboxymethylcellulose sodium, while piglets in the DQ-PIC group were orally administered with 0.5% carboxymethylcellulose sodium solution supplemented with PIC at a dosage of 80 mg·kg^-1. Piglets in DQ-CON and DQ-PIC groups were challenged with DQ at a dosage of 10 mg·kg^-124 h before slaughter, while the piglets in CON group were treated with an equal sterile saline. This treatment protocol trial lasted for 8 days. The damage of jejunum, the activity of antioxidant enzymes and the expression of genes were determined after slaughter at 36-day-old. The results showed that:1) Compared with the CON group, plasma diamine oxidase (DAO) contents of piglets in the DQ-CON group significantly increased (P<0.05), and the expression of jejunal tight junction (OCLN) gene significantly decreased (P<0.05); Compared with the DQ-CON group, plasma DAO contents of piglets in the DQ-PIC group significantly decreased (P<0.05), and the expression of OCLN gene significantly increased (P<0.05). 2) Compared with the CON group, the total superoxide dismutase (T-SOD) activity, catalase (CAT) activity and the relative expression of superoxide dismutase 1 (SOD1) significantly decreased (P<0.05), and the content of malondialdehyde (MDA) significantly increased (P<0.05). Compared with the DQ-CON group, the jejunal mucosal T-SOD activity, CAT activity, SOD1 gene and HO1 gene relative expression levels of piglets in the DQ-PIC group significantly increased (P<0.05), while the MDA content significantly decreased (P<0.05). Therefore, piceatannol can alleviate the oxidative stress damage of the piglet's jejunum induced by diquat and improve the antioxidant capacity of the jejunum and promote the restoration of intestinal morphology and barrier function.