基于iTRAQ技术挖掘猪肾组织高原低氧适应性关键蛋白

旨在探究高原低氧环境下藏猪与大约克猪肾组织中低氧适应的差异表达蛋白(differentially expressed protein,DEPs).本试验选择藏猪与大约克猪两个品种无亲缘关系的去势公猪,饲养于海拔3000 m的高原,按自由采食方式饲养至180日龄,每个品种随机选择生长情况相近的9头猪采集肾组织.利用同位素...

Mining Key Proteins of High Altitude Hypoxic Adaptability in Porcine Kidney Tissue Based on iTRAQ Technology

The aim of this study was to explore the differentially expressed proteins(DEPs) of hypoxic adaptation in kidney tissues of Tibetan pigs and Yorkshire pigs under high altitude hypoxic environment. Unrelated castrated boars from Tibetan pig and Yorkshire pig were selected as experimental animals, and fed ad libitum on the plateau with altitude of 3 000 m, up to 180-day-old, 9 pigs with similar growth and body condition were randomly selected from each breed to collect kidney tissue. In this study, isobaric tags for relative and absolute quantification(iTARQ) technique was used to analyze the difference of protein expression in kidney tissues between Tibetan pigs and Yorkshire pigs, the differentially expressed proteins were analyzed by GO functional annotation and KEGG pathway. The results showed that, a total of 4 370 proteins were obtained from kidney tissues of two pig breeds, FC>1.2 or <0.833 and P<0.05 as the criterion, a total of 181 DEPs were screened out, among them, 138 proteins were up-regulated and 43 proteins were down-regulated in the Tibetan pigs. In the functional analysis, DEPs were mainly enriched in items and pathways related to hypoxic adaptation, such as HIF-1 signaling pathway, PPAR signaling pathway, complement and coagulation cascades signaling pathway, oxidoreductase activity, carbon metabolism, regulation of blood pressure, mitochondria, etc., after further comprehensive analysis, 5 important DEPs related to hypoxic adaptation were screened out(CD59, A2M, eNOS, CDKN1B and PGK1). These DEPs play an important role in maintaining hemodynamics, regulating energy metabolism and physiological environment homeostasis, the result of this study provides a theoretical basis for further study on the adaptability of pigs to hypoxic at high altitude.