PRRSV感染仔猪肾组织的超微病理变化分析

猪繁殖与呼吸综合征病毒(PRRSV)是能引起仔猪高死亡率的疾病,给养猪业带来极大的经济损失.本研究对PRRSV感染仔猪肾组织的病毒定位及产生的超微病理变化进行观察,以期了解PRRSV对肾组织细胞以及血液循环的影响.主要采用透射电镜技术结合血清生化检测、免疫组织化学染色(IHC),对仔猪感染PRRSV后肾组织进行超微病理...

Ultrastructural Changes in Renal Tissue of Piglets Infected with PRRSV

Porcine reproductive and respiratory syndrome virus (PRRSV) is a disease that causes high piglet mortality and brings great economic loss to the pig industry. In this study, the localization and ultrastructure changes of virus in piglets’ kidney infected with PRRSV were observed to understand the effect of PRRSV on renal tissue cells and blood circulation. Transmission electron microscopy (TEM) combined with serum biochemical test and immunohistochemistry (IHC) was used to observe and analyze the renal tissue of piglets infected with PRRSV. It was found that PRRSV infection caused renal tissue damage, and the virus was mainly distributed in the cytoplasm of glomeruli, necrotic renal tubular epithelial cells and macrophages. Extensive fusion of podocyte processes or formation of microvilli and swelling of endothelial cells were found. The mitochondria in the renal tubules epithelial cells were swollen, cristae fractured and dissolved; In interstitial, inflammatory cell infiltrated. According to the observation results, PRRSV caused serious damage to the kidney, podocyte process fusion resulted in increased glomerular permeability and the injury of glomerular vascular endothelial cells leaded to microthrombus formation. These results indicated that PRRSV affected the glomerular filtration rate and blood circulation system of piglets. It provided a theoretical basis for elucidating the pathogenic mechanism of PRRSV infection.