| 布莱凯特黑牛CB1基因克隆测序及表达分析 |
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| 引用本文： | 刘贤勋,刘瑞莉,吴磊,柏学进,于堃,肖超柱,董雅娟. 布莱凯特黑牛CB1基因克隆测序及表达分析[J]. 畜牧与兽医, 2021, 0(3): 7-12 |
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| 作者姓名： | 刘贤勋 刘瑞莉 吴磊 柏学进 于堃 肖超柱 董雅娟 |
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| 作者单位： | 青岛农业大学动物科技学院动物胚胎工程中心;山东省黑牛繁育工程技术研究中心;山东布莱凯特黑牛科技股份有限公司 |
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| 基金项目： | 山东省现代农业产业技术体系牛产业创新团队建设(668/2216030)。 |
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| 摘 要： | 本试验旨在研究布莱凯特黑牛CB1基因CDS区序列和表达情况,探讨CB1基因与脂肪沉积的关系.运用RT-PCR技术克隆CB1基因CDS区序列,并进行生物学分析;运用qRT-PCR技术检测不同组织的mRNA相对表达量;以及运用免疫组化技术对蛋白进行定位分析,运用Western blot技术检测CB1蛋白在不同组织的表达量....
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| 关 键 词： | CB1基因 布莱凯特黑牛 生物信息学 荧光定量PCR 免疫组化 免疫印迹 |
Cloning,sequencing and expression of CB1 gene in Black cattle |
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| LIU Xianxun,LIU Ruili,WU Lei,BAI Xuejin,YU Kun,XIAO Chaozhu,DONG Yajuan. Cloning,sequencing and expression of CB1 gene in Black cattle[J]. Animal Husbandry & Veterinary Medicine, 2021, 0(3): 7-12 |
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| Authors: | LIU Xianxun LIU Ruili WU Lei BAI Xuejin YU Kun XIAO Chaozhu DONG Yajuan |
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| Affiliation: | (Animal Embryo Engineering Center,College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;Shandong Black Cattle Breeding Engineering Technological Research Center,Qingdao 266109,China;Shandong Black Cattle Science Technology Co.,Ltd.,Zibo 256306,China) |
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| Abstract: | This experiment was to study the sequence and expression of the CDS region of the CB1 gene in Black cattle,and to explore the relationship between the CB1 gene and fat deposition.In this test,the sequence of the CDS region of the CB1 gene was cloned using RT-PCR technology,and biological analysis was performed.The relative expression of mRNA in different tissues was detected using qRT-PCR;and the protein was analyzed immunohistochemically using Western blot.The expression of CB1 protein in different tissues was detected.The results showed that the CDS of CB1 obtained by cloning was 1495 bp,encoding 477 amino acids.The phylogenetic tree showed that the CB1 gene of Black cattle had the highest homology with that of Bos taurus,goat,and sheep(>98%).qRT-PCR results showed that CB1 was expressed in different tissues,and the relative expression was highest in the adipose tissue.Immunohistochemistry showed that CB1 was mainly expressed on the cell membrane in the adipose tissue.CB1 protein was expressed in the adipose tissue and muscle tissue.The expression level in the adipose tissue was significantly higher than that in the muscle tissue.In summary,the present research suggested that the CB1 gene was involved in lipid metabolism-related processes,providing a reference for molecular selection of meat and beef quality traits. |
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| Keywords: | CB1 gene Black cattle bioinformatics qRT-PCR immunohistochemistry Western blot |
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