一株分离自水稻种子的真菌的鉴定及系统进化分析

真菌病害是制约水稻高产稳产的重要障碍因素之一.为了研究水稻种子中真菌发生情况,对分离自水稻种子中的一株真菌进行系统发育分析及功能评价.在形态学鉴定基础上,以通用引物ITS1/ITS4对目标真菌序列进行PCR扩增、序列对比分析、邻接法(NJ)构建系统发育树.通过菌落培养特征、孢子形态特征观察,分离菌株S-51的形态特征与...

A Fungus from Rice: Species Identification and Phylogenetic Analysis

Fungal disease is one of the important obstacles for high and stable yield of rice. In order to study the occurrence of fungi in rice seeds, the phylogenetic analysis and functional evaluation of a fungus isolated from rice seeds were carried out. On the basis of morphological identification, the target fungal sequence was amplified by PCR with ITS1/ITS4, and the phylogenetic tree was constructed by neighbor-joining (NJ) and analyzing the sequence. The morphological characteristics of strain S-51 were very similar to those of Microdochium nivale in the literature, and the sequence alignment showed that the target fungi were similar to M. nivale, and the similarity rate was 100%. M. nivale and M. musae had the closest genetic relationship on the same branch as the strain of snow mold leaf blight. Sequence analysis and phylogenetic tree construction based on ITS fragments and combined with morphological characteristics could identify S-51 strain isolated from rice seeds as M. nivale.