苦皮藤内生真菌Hd3菌株抑菌活性成分分离及结构鉴定

根据活性追踪原理,采用硅胶柱层析以及高效液相色谱等技术对苦皮藤内生真菌Hd3菌株发酵液中的抑菌活性成分进行了分离纯化,得到5个具有抑菌活性的化合物,经质谱和核磁共振波谱技术分别鉴定为3'-氯代杀锥曲菌素、曲地酸、曲地酸甲酯、4',6'-二氯代曲地酸甲酯和 4'-氯代曲地酸甲酯。它们在500 mg/L的浓度下对玉米弯孢菌Curvularia lunata的孢子萌发抑制率分别为100%、67.6%、17.3%、29.1%和 30.4%,其中化合物3'-氯代杀锥曲菌素和曲地酸还对枯草芽孢杆菌Bacillus subtilis具有强烈的抑制作用。

Isolation and Identification of the Antibacterial Composition from theFermentation Broth of Endophytes in Celastrus angulatus (Strain Hd3)

Five antibacterial compounds were isolated from the fermentation broth of endophytes in Celastrus angulatus (strain Hd3) through silica gel columns and HPLC by bioassay-guided fractionation.These chemicals were elucidated as 3'-chlorotrypacidin,asterric acid, methyl asterrate,methyl dichloroasterrate and methyl chloroasterrate by NMR and MS.The bioassay results showed that the inhibition rates of 5 compounds against spore germination of Curvularia lunata were 100％,67.6％,17.3％,29.1％,30.4％ at the concentration of 500 mg/L, respectively;and 3'-chlorotrypacidin and asterric acid showed strong antibacterial activity on Bacillus subtilis.