Size Characterisation of Glutenin Polymers by HPSEC-MALLS |
| |
| Affiliation: | 1. Department of Biology, Faculty of Science, Arak University, Arak, Iran;2. Fatemeh Zahra Infertility and Reproductive Health Research Centre, Health Research Institute, Babol University of Medical Sciences, Babol, Iran;1. Zuckerberg Institute for Water Research, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus, 84990, Israel;2. Jülich Centre for Neutron Science JCNS-FRM II, Outstation at FRM II, D85747 Garching, Lichtenbergstrasse 1, Germany;3. Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel;4. Technische Universität München, Forschungs-Neutronenquelle Heinz Maier-Leibnitz (FRM II), D-85748 Garching, Germany;1. Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, Taiwan;2. Department of Biotechnology, Asia University, Wufeng, Taichung 413, Taiwan;3. Department of Biomedical Informatics, Asia University, Wufeng, Taichung 413, Taiwan;4. School of Chinese Medicine, China Medical University, Taichung 404, Taiwan;1. School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, Hunan Province, China;2. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;1. Department of Environmental Engineering, Konkuk University, 120 Neungdong-ro, Hwayang-dong, Gwangjin-gu, Seoul, Republic of Korea;2. Hanwha Environmental Research Institute, 6 Shinsung-dong, Yusung-gu, Daejon 305-345, Republic of Korea |
| |
| Abstract: | The use of an on-line coupling of a new high-performance size exclusion chromatography (HPSEC) phase characterised by a very high exclusion limit and a multiangle laser light scattering detection (MALLS) for the size characterisation of wheat glutenin polymer was examined. Flour glutenin polymers which were extracted and purified by differential solubility in aqueous 50 and 70% propan-1-ol were solubilised with or without sonication in combination with a surfactant, 2% SDS. The glutenin polymers of the two genotypes studied, Soissons (5+10, Glu-1D) and Thésée (2+12, Glu-1D), were characterised by large polydispersity and by significant differences of size distribution of total polymers. The molecular size distribution of the total polymers, which can be used to differentiate the two genotypes, was highly correlated with the percentage of high-molecular glutenin subunits (HMW-GS) in glutenins. Furthermore, the results obtained for both the SDS-soluble and SDS-insoluble glutenin polymers from the two varieties revealed significant differences in size distribution, and also in molecular conformation (dependence of size upon mass). The molecular dimensions of SDS-insoluble polymers increased more slowly with the molecular weight than SDS-soluble polymers, which suggested a more compact structure. To conclude, the method appears to be a viable procedure to obtain rapid size characterisation of unreduced glutenin. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
