Fluctuations of formamide hydrolase activity in a barley field soil after drying and rewetting |
| |
| Institution: | 1. State Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, PR China;2. Wheat Research Center of Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;1. Laboratory of Environmental Researches and Nanotechnology Development, Centro Universitário La Salle, Mestrado em Avaliação de Impactos Ambientais, Victor Barreto, 2288 Centro, 92010-000 Canoas, RS, Brazil;2. Institute of Environmental Assessment and Water Research (IDÆA-CSIC), C/Luis Solé y Sabarís s/n, 08028 Barcelona, Spain;3. Departamento Nacional de Produção Mineral (DNPM), Washington Luiz, 815, Centro, 90010-460 Porto Alegre, RS, Brazil;1. Soil Science Department, Urmia University, Urmia, Iran;2. Department of Agronomy, Faculty of Agriculture, Urmia University, Urmia, Iran;3. Department of Agricultural Development, Democritus University of Thrace, Orestiada, Greece |
| |
| Abstract: | Formamide hydrolase activity in a barley field soil was described by a two-component, rather than a one-component model of Michaelis–Menten kinetics. The two-component model had both a high-affinity (Km values of 0.5–1.0 mM) and a low-affinity (Km values of 30–60 mM) activity. Rapid and transient increases in both the overall heterotrophic activity (CO2 production) and the high-affinity component of formamide hydrolase activity were observed in rewetted soil samples, suggesting that the high-affinity component represented enzyme activity of active microorganisms. Short-term field variations in the high-affinity component during a barley growth season were consistent with rapid, transient responses of microbial activity to precipitation and soil wetting. An assay of the high-affinity component of formamide hydrolase activity as approximated by rate determination at a single low (1 mM) formamide concentration can provide an easy and rapid indication of enzyme activity related directly to active microorganisms in the soil. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
