菠萝皮渣生产优质高菌体蛋白饲料发酵菌种的筛选及发酵条件的优化

分别以热带假丝酵母、产朊假丝酵母、绿色木霉、黑曲霉为出发菌株对菠萝皮渣生产优质高菌体蛋白饲料进行发酵优良菌种的筛选试验,以发酵产物中的粗蛋白含量为考核指标,筛选出1~2株优势菌株;将筛选出的优势菌株按一定比例混合使用,对菠萝皮渣进行固态发酵生产菌体蛋白饲料,研究了接种量、料液比、温度、发酵时间等对发酵产物理化品质及感官指标的影响。结果表明,绿色木霉和产朊假丝酵母为试验的较优菌种;将绿色木霉与产朊假丝酵母1∶1体积比混合种子液使用对菠萝皮渣进行固态发酵:混合菌种总接种量10%,料液比1∶3,发酵温度30℃,发酵时间96 h为最优发酵条件。此工艺条件下,菠萝皮渣发酵所得的蛋白饲料产物的粗蛋白质含量最高、气味也比较好。

Fermentation Strains Selection and Fermentation Paraments Optimization of Protein Feed from Pineapple Pomace

To improve the content of crude ptotein in pineapple pomace,Candida tropicalis,Candida utilis,Trichoderma viride and Aspergillus niger were used as the starting strains to ferment pineapple pomace in this reserch from the above four strains.Using the crude protein content in the fermentation product as an indicator,one or two dominant strains were selected.The inoculum size,water-material ratio,fermentation temperature and fermentation time were important fermentation conditions in the fermentation process.The selected strains were mixed in a certain proportion to ferment pineapple pomace and carried out the optmiazation of the important fermentation conditions according to single factor experiment.The optimal fermentation conditions were obtained as followed:Trichoderma viride and Candida utilis were the best strains for this experiment;Trichoderma viride and Candida utilis were used as a mixed bacterial solution for the pineapple pomace fermentation in 1∶1 volume ratio:10%(V/W)inoculum size of the mixed bacterial solution,material water ratio 1∶3,fermentation temperature at 30℃and cultivation for 96 h.Under the optimiazed conditions of the process,the fermented product had the highest crude protein content and pleasant flavor.