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| 野生稻细胞核DNA提取的研究 | |
| 引用本文： | 侯思名,张薇,白现广,钟巧芳,高月芬,黄兴奇,程在全.野生稻细胞核DNA提取的研究[J].西南农业学报,2009,22(5). |
| 作者姓名： | 侯思名 张薇 白现广 钟巧芳 高月芬 黄兴奇 程在全 |
| 作者单位： | 1. 云南省农科院生物技术与种质资源研究所,云南,昆明,650223;昆明学院生命科学与技术系,云南,昆明,650118 2. 云南省农科院生物技术与种质资源研究所,云南,昆明,650223;云南大学生命科学学院,云南,昆明,650091 3. 云南省农科院生物技术与种质资源研究所,云南,昆明,650223 4. 云南南省陆良县蚕桑站,云南,陆良,655600 |
| 基金项目： | 国家自然科学基金项目 |
| 摘 要： | 药用野生稻是中国最具利用价值的野生稻资源之一,具有许多优良的性状和有利基因,是改良栽培稻培育新品种的宝贵基因库,而其CC基因组的优良基因有望以大片段形式通过BIBAC等载体转化到栽培稻中.BIBAC文库不仅能作为大片段基因组文库的载体,而且能通过根癌农杆菌介导将克隆片段导入植物基因组直接进行转化,可用于筛选分离基因等研究.如何获得基因组DNA大片段非常关键,本研究采取 琼脂糖包埋基因组的方法获得大片段DNA,并对其中的一些步骤进行了优化,为构建药用野生稻BIBAC文库打下了很好的基础,值得在其它植物类似研究中参考. |
| 关 键 词： | 药用野生稻 BIBAC载体 基因组大片段DNA制备 |
Research on Efficient Extraction of Genome DNA of Oryza officinalis |
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| HOU Si-ming,ZHANG Wei,BAI Xian-guang,ZHONG Qiao-fang,GAO Yue-fen,HUANG Xing-qi,CHENG Zai-quan.Research on Efficient Extraction of Genome DNA of Oryza officinalis[J].Southwest China Journal of Agricultural Sciences,2009,22(5). | |
| Authors: | HOU Si-ming ZHANG Wei BAI Xian-guang ZHONG Qiao-fang GAO Yue-fen HUANG Xing-qi CHENG Zai-quan |
| Abstract: | Oryza officinalis Wall.ex Watt is one of the most valuable wild rice resources in China. It contains a lot of specific and helpful genes for the further improvement of cultivated rice. Some excellent genes existing in O. officinalis were possibly transferred into some cultivated rice through BIBAC (binary bacterial artificial chromosome,BIBAC) vector in the form of large fragments. BIBAC vector was one of the efficient vectors in gene cloning and transformation in plants. The key to construct genome library was to obtain mega-fragments. The way that mega-fragments were embedded in agarose gel was used and optimized in this study,which could provide a solid foundation to construct the BIBAC library of O. officinalis. |
| Keywords: | Oryza officinalis BIBAC vector Extraction of genome large fragment |
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