仔猪O型口蹄疫母源抗体消长试验

目的]为制定科学合理的仔猪口蹄疫免疫程序提供依据。方法]以规模化养猪场和散养户中经猪口蹄疫O型灭活疫苗免疫后母猪所生产的仔猪为研究对象,利用液相阻断ELISA诊断试剂盒对仔猪母源抗体消长情况进行检测。结果]仔猪通过母乳获得被动保护,在21日龄母源抗体达到高峰,规模化猪场和散养户母猪的平均效价分别达1∶142和1∶89。规模化猪场场仔猪21~30日龄保护率在80%以上,45日龄下降到65%,60日龄时基本无保护;而农户仔猪21日龄保护率为60%左右,30日龄仅为40%左右。农户饲养母猪免疫抗体水平远低于规模化猪场。结论]对规模化猪场的仔猪进行口蹄疫疫苗的最佳首免时间为45~50日龄,而对散养户仔猪的最佳首免时间应为30日龄左右。

Test on Maternal Antibody Dynamics against Foot and Mouth Disease Type O in Piglets

Objective] The research aimed to provide basis for establishing scientific and reasonable piglet immune procedure against mouth and foot disease.Method] The piglets produced by sows that were immunized with the inactivated vaccine against swine mouth and foot disease in large-scale pig farms and scatter-feed households were taken as research objects.And the dynamic conditions of maternal antibody in piglets were detected by using solution-phase blocking ELISA diagnostic kit.Result] Piglets obtained the passive protection through breast milk and the maternal antibody reached its peak at the age of 21 days.Then the average tiers of sows in large-scale pig farms and scatter-feed households reached 1∶142 and 1∶89 resp.The protection rate of the piglets in large-scale pig farms were more than 80% at the age of 21～30 days and decreased to 65% at the age of 45 days.And the piglets were basically not protected at the age of 60 days.The protection rate of the piglets in farm households was about 60% at the age of 21 days and was only about 40% at the age of 30 days.The immune antibody level of sows bred in farm households was lower than that in large-scale pig farms.Conclusion] The optimum first immune time for immunizing the piglets in large-scale pig farms with foot and mouth disease vaccine was at the age of 45～50 days and that for the piglets in scatter-feed households should be at the age of 30 days.