| Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F1 of Cultivated Rice （Oryza sativa L.） Using SSR Markers |
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| 作者姓名： | YAO Yan LU Yong-gen LIU Xiang-dong FENG Jiu-huan ZHANG Gui-quan |
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| 作者单位： | Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China |
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| 基金项目： | This paper is translated from its Chinese version in Scientia Agricultura Sinica, This work was supported by the National Natural Science Foundation of China （39970048）, Guangdong Provincial Natural Science Foundation （990707） and the Fok Ying Tung Education Foundation （71021）. |
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| 摘 要： | S-a, S-b and S-c are three loci for F1 pollen sterility in cultivated rice （Oryza sativa L.）. Taichung 65 （T65） is all Sj/Sj at these three loci, while its F1 pollen sterile near-isogenic lines, TISL2 （S-b）, TISL4 （S-a） and TISL5 （S-c） is Sj/Sj according to their respective sterility locus. Using SSR molecular marker to detect the segregation of the allele Si and Sj in pollen calli population induced from different hybrid F1, which have different pollen sterility locus, showed that the segregation of allele Si and Sj was distorted. The distorted direction of pollen calli population in vitro was not the same as F2 population in vivo. The quantities of pollen callus carrying Sj were much more than that of carrying Siat S-a and S-c locus, the ratio of Si and Sj were 1：4.81 and 1：1.96 respectively. But the opposite tendency was observed at S-b locus, the ratio of Si and Sj being 1：0.35. At the same time, all these results were undisturbed by either culture medium or culture period.
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| 关 键 词： | 水稻 分子标记 基因类型 花粉 |
Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers |
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| YAO Yan LU Yong-gen LIU Xiang-dong FENG Jiu-huan ZHANG Gui-quan.Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers[J].Agricultural Sciences in China,2006,5(6):412-416. |
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| Authors: | Yan YAO Ph D Associate Professor Yong-gen LU Xiang-dong LIU Jiu-huan FENG Gui-quan ZHANG |
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| Institution: | aGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R. China |
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| Abstract: | S-a, S-b and S-c are three loci for F1, pollen sterility in cultivated rice (Oryza sativa L.). Taichung 65 (T65) is all Sj/Sj at these three loci, while its F1 pollen sterile near-isogenic lines, TISL2 (S-b), TISL4 (S-a) and TISL5 (S-c) is Si/Si according to their respective sterility locus. Using SSR molecular marker to detect the segregation of the allele Si and Sj in pollen calli population induced from different hybrid F1, which have different pollen sterility locus, showed that the segregation of allele Si and Sj was distorted. The distorted direction of pollen calli population in vitro was not the same as F2 population in vivo. The quantities of pollen callus carrying Sj were much more than that of carrying Si at S-a and S-c locus, the ratio of Si and Sj were 1:4.81 and 1:1.96 respectively. But the opposite tendency was observed at S-b locus, the ratio of Si and Sj being 1:0.35. At the same time, all these results were undisturbed by either culture medium or culture period. |
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| Keywords: | rice (Oryza sativa L ) pollen sterility pollen callus distorted segregation SSR marker |
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