| 樟树总DNA提取技术的研究 |
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| 引用本文： | 潘晓华,黄儒珠,朱锦懋.樟树总DNA提取技术的研究[J].福建林业科技,2004,31(1):31-33,41. |
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| 作者姓名： | 潘晓华 黄儒珠 朱锦懋 |
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| 作者单位： | 福建师范大学生物工程学院,福建,福州,350007 |
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| 基金项目： | 福建省自然科学基金资助项目(B0110013) |
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| 摘 要： | 比较了常规的CTAB法与改进的CTAB法对樟树总DNA提取的结果。在常规的CTAB法基础上,采取先破碎细胞收集细胞核,将存在于细胞质中的大量影响DNA提取质量的多酚等次生代谢物除去后,再加核裂解液释放DNA,经分离纯化,提得的DNA通过A260/A280比值的测定及电泳,限制性内切酶消化,PCR扩增,结果表明,用改进的CTAB法提取的DNA纯度和完整性均优于常规的CTAB法。
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| 关 键 词： | 樟树 DNA提取 方法 |
| 文章编号： | 1002-7351(2004)01-0031-03 |
Study on Total DNA Extraction from Cinnamomun Camphora |
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| PAN Xiao-hua,HUANG Ru-zhu,ZHU Jin-mao.Study on Total DNA Extraction from Cinnamomun Camphora[J].Journal of Fujian Forestry Science and Technology,2004,31(1):31-33,41. |
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| Authors: | PAN Xiao-hua HUANG Ru-zhu ZHU Jin-mao |
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| Abstract: | A comparative study on total DNA extraction from Cinnamomun Camphora which is rich secondary metabolites.Based on the conventional CTAB methods,an efficient procedure was developed.The major improvement included elimination a lot of secondary metabolites such as polyphenols etc,and collection of nuclei before free DNA.Then CTAB-free buffer was added to extract total DNA.The results from ratios of A_(260) and A_(280),electrophoresis,digestion with restriction endonucleases and PCR amplification suggested that the modified CTAB methods produce high quality DNA compared with conventional CTAB methods. |
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| Keywords: | Cinnamomun Camphora DNA extraction method |
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