反相高效液相色谱法同时测定金银花中的绿原酸和木犀草苷方法的优化 |
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引用本文: | 刘文静,潘葳,涂杰峰,杨继伟.反相高效液相色谱法同时测定金银花中的绿原酸和木犀草苷方法的优化[J].安徽农业科学,2014(26):8948-8950,8953. |
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作者姓名: | 刘文静 潘葳 涂杰峰 杨继伟 |
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作者单位: | 1. 福建省农业科学院中心实验室,福建福州350003;福建省精密仪器农业测试重点实验室,福建福州350003 2. 福建省科技厅农牧业科研中试中心,福建福州,350001 |
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基金项目: | 福建省农业科学院重大专项子专题3 |
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摘 要: | 目的]研究反相高效液相色谱法同时测定金银花中的绿原酸和木犀草苷的优化色谱条件.方法]采用反相高效液相色谱法(RP-HPLC)同时测定金银花中绿原酸和木犀草苷含量,并对色谱条件进行优化.结果]最佳色谱条件为色谱柱Waters SunFireTMC18(4.6mm×150 mm,3.5 μm)、柱温40℃,流动相为乙腈-0.5%冰醋酸,梯度洗脱为0~15 min(10:90~20:80)、16 ~ 17 min(20:80 ~ 10:90),流速1.0 ml/min,检测波长0~15 min 328 nm、16 ~ 17 min 350 nm.结论]优化后缩短了测试时间,为金银花质量的快速检测提供参考依据.
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关 键 词: | 反相高效液相色谱法 金银花 绿原酸 木犀草苷 优化 |
Optimization of the Determination Method of the Chlorogenic Acid and Luteolin-7-O-glucoside in Flos Lonicerae by RP-HPLC |
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Institution: | LIU Wen-jing;PAN Wei;Central Laboratory,Fujian Academy of Agricultural Sciences;Fujian Key Laboraotory of Precision Measurement of Agriculture; |
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Abstract: | The research aimed to study the optimal chromatographic conditions of the deter mination method of the ehlorogenie acid and luteolin-7-O-glueoside in flos lonieerae by RP-HPLC. Method] A RP-HPLC method with UV detector was established and optimized to de- ter mine the ehlorogenie acid and luteolin-7-O-glueoside in Flos Lonieerae. Result ] The chromatographic conditions were: Waters SunFireT- MCI8 (4.6 mmx 250 mm, 3.5 p,m) used for column( at 40 ~C ) with 0.5% acetic - acetonitrile. Linear gradient elution: 0 - 15 rain( 10:90 - 20 : 80), 16 - 17 min(20:80 ~ 10: 90), flow rate :1 ml/min, deterction wavelength:0 - 15 rain 328 nm, 16 - 17 rain 350 nm. Conclusion] After optimization, the test time was shortened. The study provides the reference for deter ruination method of rapid detection of flos lonicerae quality. |
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Keywords: | RP-HPLC Flos lonicerae Chlorogenic acid Luteolin-7-O-glucoside Optimization |
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