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山药总皂甙的提取研究
引用本文:黄桂东,钟先锋,吴龙奇. 山药总皂甙的提取研究[J]. 农产品加工.学刊, 2006, 0(11): 42-45
作者姓名:黄桂东  钟先锋  吴龙奇
作者单位:河南科技大学,食品科学与生物工程学院,河南,洛阳,471003
摘    要:以山药中总皂甙提取率为考察指标,应用单因素试验和正交设计对山药总皂甙提取工艺中的浸提温度、乙醇的体积分数、浸提时间、料液比进行了研究。以薯蓣皂甙为标准,香草醛—高氯酸为显色剂,冰醋酸为溶剂,用分光光度计在波长为544nm处对山药总皂甙的含量进行了测定,得出山药总皂甙的最佳提取工艺条件是:浸提温度为60℃,浸提时间为6h,乙醇体积分数为80%,料液比为1∶8。

关 键 词:山药  皂甙  提取  分光光度法
文章编号:1671-9646(2006)11-0042-04
收稿时间:2006-09-08
修稿时间:2006-09-08

Study on Extraction of the Total Saponins of Chinese Yam
Huang Guidong,Zhong Xianfeng,Wu Longqi. Study on Extraction of the Total Saponins of Chinese Yam[J]. Nongchanpin Jlagong.Xuekan, 2006, 0(11): 42-45
Authors:Huang Guidong  Zhong Xianfeng  Wu Longqi
Abstract:A method for the determination of total saponins of Chinese yam was established in this paper. The dioscin was used as a standard compound,the vanillin-perchloric acid as chromogenic agent and glacial acetic acid as solvent. The extraction technique of saponins from Chinese yam was studied by spectrometric method. Extracting temperature, extracting time,ethanol concentration and the ratio of raw material and water were selected as four factors to design the orthogonal test,and the optical condition of extraction was obtained. The results showed that the optical condition of extraction is as following:extracting temperature 60 ℃,extracting time 6 h,ethanol concentration 80%,and the ratio of raw material and water 1∶8.
Keywords:chinese yam   saponins   extraction    spectrometric method
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