蛇龙珠葡萄酒酒龄花青素高效液相色谱（HPLC）指纹图谱研究

【目的】建立葡萄酒酒龄花青素HPLC指纹图谱，区分鉴定不同酒龄（年份）蛇龙珠葡萄酒。【方法】HPLC检测：采用反相C18柱，调整流动相pH 1.6，二元梯度洗脱，检测波长518 nm。指纹图谱建立方法：计算HPLC色谱峰的相对保留时间和相对峰面积，按相对保留时间排列相对应的相对峰面积，并编号。【结果】利用14个主要的色谱峰建立了蛇龙珠葡萄酒酒龄花青素HPLC指纹图谱，并根据资料对葡萄酒各色谱峰成分进行了初步的定性鉴定。利用HPLC方法分离出14号峰，该峰显著影响葡萄酒酒龄花青素指纹图谱。随酒龄的增加色谱峰相对面积出现显著变化。6个不同酒龄（年份）葡萄酒的花青素指纹图谱明显不同。【结论】试验结果稳定，效果好；花青素HPLC指纹图谱可以区分不同酒龄葡萄酒，是区分鉴定葡萄酒酒龄可行的方法。

Identification of Cabernet Gernische Wine Age by High-Performance Liquid Chromatography Anthocyanins Fingerprint

【Objective】 The anthocyanin HPLC fingerprints of aging wines and identification of 6 different vintage of Cabernet Gernische wines were established. 【Method】HPLC analysis: Chromatographic condition was RP-C18 column, pH 1.6 in mobile phases, binary solvent delivery gradient elution, and UV-Vis detection was used at 518 nm. Fingerprint: The relative reserving time and relative area of chromatography peaks were computed, and the relative area of chromatography peaks were arranged according to relative reserving time in turn. 【Result】14 main peaks were separated and used in fingerprints. The chromatography peaks were identified preliminaryly. By using HPLC method, for the first time, the fourteenth peak was separated and it was found that the relative content of this peaks was relevant to the aging of wine closely. The anthocyanin HPLC fingerprints had a big difference from different vintage Cabernet Gernische wines. 【Conclution】 The good reproducibility and stability were shown in this experiment. The method of anthocyanin HPLC fingerprints is feasible for distinguishing the age (vintage) of red wine.