Identification of larval-stage antigens of ascaris suum recognized with immune sera from pigs. |
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| Authors: | H Kasug-Aoki N Tsuji K Suzuki T Arakawa Y Matsumoto T Isobe |
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| Institution: | Laboratory of Parasitic Diseases, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. |
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| Abstract: | Antigens were identified from the third-stage larvae (L3) and lung-stage larvae of Ascaris suum by two-dimensional immunoblot method with antisera obtained from pigs that received chemically abbreviated Ascaris suum larval infections. Forty-seven and 13 spots were recognized as antigens from the L3 and lung-stage larvae, respectively. Their apparent molecular weight ranged from 20 to 101 kDa and their isoelectric point from 3.6 to 8.0. The present study provides a framework for further molecular cloning of those antigens and consequently leads to the development of recombinant peptide vaccines against A. suum. |
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