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| 涪陵水牛mtDNA D-loop区遗传多样性研究 | |
| 引用本文： | 谢文美,苏锐,李明晖,吴平,姚福吉,卢群志,陈宏,雷初朝. 涪陵水牛mtDNA D-loop区遗传多样性研究[J]. 西北农业学报, 2008, 17(5): 56-60 |
| 作者姓名： | 谢文美 苏锐 李明晖 吴平 姚福吉 卢群志 陈宏 雷初朝 |
| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100 2. 西南大学动物科技学院,重庆北碚,400716 3. 重庆市南川区畜牧兽医局,重庆南川,408400 4. 西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100;徐州师范大学细胞与分子生物学研究所,江苏徐州,221116 |
| 基金项目： | 教育部跨世纪优秀人才培养计划，西北农林科技大学校科研和教改项目 |
| 摘 要： | 利用测序技术和生物信息学分析技术,对涪陵水牛27个个体的线粒体DNA D-loop 915 bp全序列的遗传多样性及系统进化进行分析,检测到16种单倍型,其核苷酸多态位点48个,约占所测核苷酸总长的5.24%,其中有43个转换,5个颠换。涪陵水牛mtDNA D-loop区核苷酸多样度(π值)为0.0196±0.0020,单倍型多样度(H)为0.9060±0.0460,表明涪陵水牛mtDNA遗传多样性丰富。根据单倍型构建了涪陵水牛的NJ分子系统树,发现存在沼泽型水牛支系A和B,表明涪陵水牛具有2个母系起源。 |
| 关 键 词： | 涪陵水牛 mtDNAD-loop 遗传多样性 单倍型 支系 |
| 收稿时间： | 2008-02-25 |
| 修稿时间： | 2008-04-11 |
Study on mtDNA D-loop Genetic Diversity in Fuling Swamp Buffalo |
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| XIE Wen-mei,SU Rui,LI Ming-hui,WU Ping,YAO Fu-ji,LU Qun-zhi,CHEN Hong and LEI Chu-zhao. Study on mtDNA D-loop Genetic Diversity in Fuling Swamp Buffalo[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(5): 56-60 | |
| Authors: | XIE Wen-mei SU Rui LI Ming-hui WU Ping YAO Fu-ji LU Qun-zhi CHEN Hong LEI Chu-zhao |
| Affiliation: | College of Animal Science and Technology, Northwest A&F University, Shaanxi Key laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Southwest University, Beibei Chongqing 400716, China;Nanchuan County Animal Husbandry and Veterinary Medicine Station of Chongqing City, Nanchuan Chongqing 408400, China;Nanchuan County Animal Husbandry and Veterinary Medicine Station of Chongqing City, Nanchuan Chongqing 408400, China;Nanchuan County Animal Husbandry and Veterinary Medicine Station of Chongqing City, Nanchuan Chongqing 408400, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China;Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou Jiangsu 221116, China;College of Animal Science and Technology, Northwest A&F University, Shaanxi Key laboratory of Molecular Biology for Agriculture, Yangling Shaanxi 712100, China |
| Abstract: | The complete mitochondrial DNA(mtDNA)D-loop sequences,915 bp in length,in 27 Fuling swamp buffalo samples from Chongqing city were analyzed.The results revealed 16 m it ochondrial haplotypes with 48 polymorphic sites,covering 5.24% of the entire length of the Dloop sequences. Among the polymorphic sites,there were 43 transit ions and 5 transversions.The nucleotide diversity (value)and haplotype diversity(H)estimated were 0.0196±0.0020 and 0.9060±0.0460,respectively,showing rich mitochondrial genetic diversity in Fuling swamp buffalo population.The constructed Neighbor Joining molecular phylog enetictree based on the 16 mt DNA Dloop haplotypes, indicated that two lineages A and B were detected which showed two maternal origins in Fuling swamp buffalo population. |
| Keywords: | Fuling buffalo Mitochondrial DNA D-loop Genetic diversity Haplotype Lineage |
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