五星黄鸡BMP15基因多态性分析 |
| |
引用本文: | 王巧,刘锦梅,张廷荣,李桢. 五星黄鸡BMP15基因多态性分析[J]. 安徽农业科学, 2013, 0(21): 8847-8849,8864 |
| |
作者姓名: | 王巧 刘锦梅 张廷荣 李桢 |
| |
作者单位: | 青岛农业大学动物科技学院,山东青岛,266109;青岛农业大学动物科技学院,山东青岛,266109;青岛农业大学动物科技学院,山东青岛,266109;青岛农业大学动物科技学院,山东青岛,266109 |
| |
基金项目: | 山东省“泰山学者”岗位建设工程专项基金.鸣谢 感谢中国农科院畜牧兽医研究所刘鸿举博士提供试验材料与研究指导. |
| |
摘 要: | [目的]分析五星黄鸡的BMPl5基因多态性。[方法]以217只五星黄鸡L系母鸡为材料,采用全测序方法检测BMPl5基因的多态性及其与300日龄产蛋数的相关性。[结果]在BMPl5基因2个外显子中共检测到13个SNPs,在外显子1中检测到4个。其中C34T突变使亮氨酸变为苯丙氨酸;在外显子2中检测到9个,其中G540A突变使甘氨酸变为丝氨酸,其他位点为无义突变。G540A位点AA基因型比GA基因型产蛋数平均多16.7枚,差异显著(P〈O.05);其余位点不同基因型产蛋数差异不显著。[结论]该研究为五星黄鸡高产蛋数的分子标记辅助选择提供了参考。
|
关 键 词: | 五星黄鸡 BMP15基因 多态性 300日龄产蛋数 关联分析 |
Analysis of Polymorphism of BMP15 Gene in Wuxing-yellow Chicken Breeds |
| |
Affiliation: | WANG Qiao et al ( College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong 266109) |
| |
Abstract: | [ Objective] The aim was to analyze the p01ymorphism of BMP15 gene in Wuxing-yellow chicken breeds. [ Method] Two hundred and seventeen laying hens of Wuxing-yellow chicken breeds were chosen for investigating the p01ymorphism of BMP15 gene by sequencing and its association with egg-laying performance in 300 days. [ Result] Thirteen SNPs were totally detected in the two exons of BMP15 gene, 4 of which were found in exonl and 9 of which were found .in exgn2. The mutation C34T in exonl resulted in an amino acid change of Leueine to Phenylalanine and the mutation G540A in exon2 resulted in a change of Glyeine to Serin. Other sites were nonsense mutation. For G540A site, the average number of egg-laying of AA genotype was 16.7 more than that of GA genotype, and there was a significant difference (P 〈 0.05 ) in egg-laying performance of hens between that two gdnotypes. The number of eggs between different genotypes of the rest had no signif- icantly difference. [ Conclusion] The study provided a reference for the molecular marker assisted selection of high number of egg-laying in Wuxing-yellow chicken breeds. |
| |
Keywords: | Wuxing-yellow chicken breeds BMP15 gene Polymorphism 300 days egg yield Association analysis |
本文献已被 CNKI 维普 万方数据 等数据库收录! |