高产漆酶菌的筛选及对污水中萘降解的研究简

目的]筛选出高产漆酶菌株,研究菌株产漆酶对污水中萘是否有降解能力.方法]采取液体发酵法和气相色谱法.结果]主要对21个隶属于担子菌的菌株进行了漆酶活性的比较.首先,平板培养进行比较,之后液体发酵的试验方法培养15 d后得到高酶活的菌株,漆酶酶活高（酶活达到1 000 U/ml以上）的菌株有3个,依次是香栓菌（Trametes suaveolens）、灰管层孔菌（Fomes lignosus）和彩绒革盖菌（Trametes versicolor）.进一步对香栓菌的漆酶性质进行研究,其最适pH为4.5,最适温度为35℃,最适碳源为乳糖,氮源为蛋白胨.通过对其中8种产漆酶菌株对污水中萘（含量）的降解处理,结果表明菌株香栓菌和硬毛栓菌（Trametea trogii）产生的漆酶对污水中的萘降解率均为100％,而一色齿毛菌（Cerrena unicolor）和烟管菌（黑管菌）（Bjerkandera adusta）对污水中的萘降解均能达到50％以上.结论]得到3株产漆酶酶活较高的菌株.菌株产漆酶对污水中萘具有一定的降解能力.

Screening of High Laccase-producing Strains and Studies on Degradation of Naphthalene in Sewage

Objective ] High laccase-producing strains were screened and the degradation of naphthalene in sewage was studied. Method ] Liquid fermentation and gas chromatography were used. Result] The laccase activity of 21 strains belonged to basidiomycetes was compared. 3 high laccase-producing level strains were screened out using the plate cultivation and fluid fermentation method, which were T. suaveolens, F. lignosus and T. versicolor. The laccase activity of the strains on 15th days were above 1 000 U/ml. Then the laccase properties of T. suave- olens was studied. The optimum pH was 4.5, the optimum temperature was 35 ~C, the optimum carbon source was lactose, and nitrogen source was peptone. The resunhs of degradation of naphthalene of the 8 strains were as follows ： the naphthalene degradation rate of T. suaveo- lens and T. trogii were all 100% , and he naphthalene degradation rate of C. unicolor and B. adusta were more than 50%. Conclusion] 3 high laccase-producing level strains were screened out, and the laccase-producing strains could degrate the naphthalene in sewage.