猪链球菌PCR检测技术研究进展

猪链球菌是猪的一种重要病原菌,并且也会引起人的链球菌病。有35个荚膜血清型(1/21、~34),通常自发病或死亡猪体分离获得1,2,7,9型和14型菌株,其中2型是毒力最强的血清型。根据已知猪链球菌16 SrRNA及溶血素(sly)、谷氨酸脱氢酶(gdh)、荚膜多糖(cps)、胞壁蛋白或溶菌酶释放相关蛋白(mrp)、胞外因子(epf)编码基因序列设计特异性引物,建立猪链球菌群和1(14),2(1/2),7型和9型特异性PCR或多重PCR,建立2型致病性菌株和1型高致病性菌株毒力鉴定PCR或多重PCR,用于检测和鉴别临床病料和细菌分离物中的猪链球菌,具有高敏感性和高特异性,与其他致病菌及其他血清的猪链球菌型无交叉反应,为疫病诊断及流行病学的研究提供了快速、简便和有用的工具。

Advance in PCR Assays for Detection of Streptococcus suis

Streptococcus suis is an important pathogen of pigs and occasionally causes serious human disease.It has thirty-five different capsular serotypes(types 1/2 and 1 through 34).Serotypes 1,2,7,9,and 14 are usually isolated from diseased or dead pigs and serotype 2 is considered to be the most-virulent serotype.Specific primers were designed and synthesized based on 16S rRNA and sly,gdh,cps,mrp,epf gene sequences of known Streptococcus suis strains.The PCR or multiplex PCR assays were developed for the detection of group and serotypes 1(plus 14),2(plus 1/2),7,9 and the discrimination of serotype 2 virulent strains and serotype 1 highly virulent strains of Streptococcus suis in clinical samples or bacterial isolates with higher sensitivity and specificity,no cross reaction with other pathogenic bacteria and other serotypes of Streptococcus suis.The PCR assays can be rapid,simple,useful tools both for diagnostic purposes and in epidemiological and transmission studies of porcine streptococosis.