| 多疣壁虎DNA含量的测定 |
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| 引用本文： | 刘炎,李静,顾云,沈宓,王勇军,刘梅.多疣壁虎DNA含量的测定[J].安徽农业大学学报,2010,37(4). |
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| 作者姓名： | 刘炎 李静 顾云 沈宓 王勇军 刘梅 |
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| 作者单位： | 江苏省神经再生重点实验室,南通大学,南通,226001;江苏省神经再生重点实验室,南通大学,南通,226001;江苏省神经再生重点实验室,南通大学,南通,226001;江苏省神经再生重点实验室,南通大学,南通,226001;江苏省神经再生重点实验室,南通大学,南通,226001;江苏省神经再生重点实验室,南通大学,南通,226001 |
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| 基金项目： | 国家自然科学基金，江苏省教育厅自然科学基金 |
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| 摘 要： | 采用流式细胞术方法测定多疣壁虎(Gekko japonicus)DNA含量(C值).结果表明,多疣壁虎的C值为2.928 Pg·C-1,计算其基因组大小约为2.86×109bp,雌性和雄性壁虎的C值分别为2.934 Pg和2.923 Pg.数据统计经独立样本t检验性别问无显著差异.相同方法测定了另外两种爬行动物北草晰(Takydromusseptentrionalis)和原尾壁虎(Hemidactylus bowringii)的C值,分别是(2.10±0.03)Pg·C-1和(2.04 ±0.03)Pg·C-1.并计算其基因组大小,北草晰为2.054×109bp,原尾壁虎为1.995×109bp.
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| 关 键 词： | 流式细胞术 DNA含量(C值) 多疣壁虎 爬行动物 |
Determination of DNA content of Gekko japonicus by flow cytometry |
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| LIU Yan,LI Jing,GU Yun,SHEN Mi,WANG Yong-jun,LIU Mei.Determination of DNA content of Gekko japonicus by flow cytometry[J].Journal of Anhui Agricultural University,2010,37(4). |
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| Authors: | LIU Yan LI Jing GU Yun SHEN Mi WANG Yong-jun LIU Mei |
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| Abstract: | The present study aims to investigate the DNA content(C-value)of Gekko japonicus by flow cytometry. The result showed that the C-value of Gekko japonicus is 2.928 Pg·C-1 with the calculated genome size approximately of 2.86×109bp.The C-values for female and male are 2.934 pg and 2.923 Pg，respectively.Independent. Samples T test indicated that the C.values of different genders have no significant difference.Meantime，we observed the C-values Qf two other reptile species，and found that C-values of Takydromus septentrionalis and Hemidactylus bowringii are(2.10±0.03)Pg·C-1 and(2.04 4-0.03)Pg·C-1.By this means the calculated approximate genome size of Takvdromus septentrionalis is 2.054×109 bp and that of Hemidactylus bowringii is 1.995×109bp. |
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| Keywords: | flow cytometry DNA content(C-value) Gekko japonicus reptile |
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