| 卵母细胞体外成熟时间对绵羊核移植效率的影响(英文) |
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| 引用本文： | 张晓建,安志兴,李学斌,王清华,范彩云,杨丽芬,张涌. 卵母细胞体外成熟时间对绵羊核移植效率的影响(英文)[J]. 农业科学与技术, 2008, 9(4): 108-111 |
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| 作者姓名： | 张晓建 安志兴 李学斌 王清华 范彩云 杨丽芬 张涌 |
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| 作者单位： | 河南科技学院;西北农林科技大学生物工程研究所 |
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| 摘 要： | [Objective] The study aimed to provide references for the time of oocyte maturation in vitro and enucleation in the course of sheep nuclear transfer(NT).[Method] Compared the effects of different maturation time of oocytes on enucleation efficiency and reconstructed embryo development by means of blind enucleation and fluorescence microscopy.[Result] Treatment of IVM(in vitro maturation)19-21 h was significantly higher than IVM 16-18 h treatment in oocyte maturation rate(P<0.05)and was significantly higher than IVM 22-24 h treatment in enucleation rate(P<0.05).Three treatments had no significant difference in cleavage rate and blastocyst rate(P>0.05),but IVM 19-21 h treatment was significantly higher than the other 2 treatments in average cell number of blastocysts(P<0.05).[Conclusion] The appropriate in vitro maturation time of oocytes was 19-21 h for sheep nuclear transfer,which could significantly improve the quality of blastocysts according to the cell number per blastocyst(P<0.05).
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| 关 键 词： | Oocyte Maturation time Sheep nuclear transfer Enucleation |
Effects of in Vitro Maturation Time of Oocytes on Sheep Nuclear Transfer Efficiency |
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| ZHANG Xiao-jian,AN Zhi-xing,LI Xue-bin,WANG Qing-hua,FAN Cai-yun,YANG Li-fen,ZHANG Yong. Effects of in Vitro Maturation Time of Oocytes on Sheep Nuclear Transfer Efficiency[J]. Agricultural Science ＆ Technology, 2008, 9(4): 108-111 |
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| Authors: | ZHANG Xiao-jian AN Zhi-xing LI Xue-bin WANG Qing-hua FAN Cai-yun YANG Li-fen ZHANG Yong |
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| Affiliation: | 1.Henan Institute of Science and Technology,Xinxiang 453003;2.Institute of Bioengineering,Northwest A&F University,Yangling 712100 |
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| Abstract: | [Objective] The study aimed to provide references for the time of oocyte maturation in vitro and enucleation in the course of sheep nuclear transfer (NT).[Method] Compared the effects of different maturation time of oocytes on enucleation efficiency and reconstructed embryo development by means of blind enucleation and fluorescence microscopy. [Result] Treatment of IVM (in vitro maturation)19-21 h was significantly higher than IVM 16-18 h treatment in oocyte maturation rate(P<0.05)and was significantly higher than IVM 22-24 h treatment in enucleation rate(P<0.05). Three treatments had no significant difference in cleavage rate and blastocyst rate(P>0.05), but IVM 19-21 h treatment was significantly higher than the other 2 treatments in average cell number of blastocysts(P<0.05). [Conclusion] The appropriate in vitro maturation time of oocytes was 19-21 h for sheep nuclear transfer, which could significantly improve the quality of blastocysts according to the cell number per blastocyst(P<0.05). |
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| Keywords: | Oocyte Maturation time Sheep nuclear transfer Enucleation |
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