Molecular diversity of Brazilian strains of porcine circovirus type 2 (PCV-2) |
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Authors: | de Castro Alessandra Marnie Martins Gomes Cortez Adriana Heinemann Marcos Bryan Brandão Paulo Eduardo Richtzenhain Leonardo José |
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Institution: | 1. National Institute for Health and Welfare (THL), Department of Health Protection, P.O. Box 310, FI-90101, Oulu, Finland;2. National Institute for Health and Welfare (THL), Department of Health Protection, Finn-Medi I, Biokatu 6, 33520 Tampere, Finland |
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Abstract: | Porcine circovirus 2 (PCV-2) is associated with a broad range of syndromes. In this study, eight pig tissue samples from two Brazilian states were analyzed using six PCR primer pairs amplifying a 1705-bp fragment of the PCV-2 genome. The NJ distance-based method was used for the phylogenetic analysis with the eight field strains herein, 15 GenBank sequences and using PCV-1 as an out-group. This yielded two major clusters (A and B) for this viral species, with the Brazilian strains segregating with European and Asian sequences. Nucleotide identity was 99.7 to 100% among the sequences. This information can be used in further studies of pathogenesis related to PCV-2 in Brazil. |
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