杨树叶片数量性状相关联标记及其图谱定位研究

利用数量遗传学方法和分子标记相结合，对美洲黑杨与青杨杂交产生的Ｆ２群体５个叶片数量性状进行了相关联标记及其图谱定位研究。用单因素方差分析检测出与叶片，叶宽，叶片／宽比，叶柄长和叶面积相关联的ＲＡＰＤ标记分别为１０、１０、４、９，１２个，联合贡献率分别为６６．２３％，６１．８２％，３２．８６％，５９．６７％，８１．７９％。

DETECTION AND IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH QUANTITATIVE TRAITS OF LEAF IN POPLAR

Both quantitative genetics method and molecular marker method were used to identify marker loci associated with 5 quantitative leaf traits in a F\-2\|population from a cross of \%Populus deltoides\% Marsh. and \%P. cathayana\% Rehd.. Though analysis of variance (ANOVA) possesses a low resolution in mapping QTLs, it is an effective method for detecting QTLs. Single\|factor analysis of variance was conducted for each locus\|trait combination to identify QTLs. The results revealed that 10,10,4,9 and 12 markers were significantly associated with leaf length, leaf width, ratio of length to width of leaf, petiole length and leaf area respectively, which accounted for 66.23%, 61.82%, 32.86%, 59.67% and 81.79% of the total phenotypic variation respectively. Two\|factor analysis of variance showed that 4,2,1,5 and 7 significant interactions among these markers were associated with leaf length, leaf width, ratio of length to width of leaf, petiole length and leaf area respectively. Most of marker loci associated with these quantitative traits were on the 4th,12th,15th and 17th line of linkage groups.