Genetic diversity and differentiation of <Emphasis Type="Italic">Sclerotinia sclerotiorum</Emphasis> populations in sunflower |
| |
Authors: | Ziqin Li Yingchun Wang Yu Chen Jinxiu Zhang W G Dilantha Fernando |
| |
Institution: | (1) College of Life Science, Inner Mongolia University, Hohhot, 010020, People’s Republic of China;(2) Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada;(3) Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada;(4) Present address: Inner Mongolia Academy of Agricultural Sciences, Inner Mongolia, People’s Republic of China |
| |
Abstract: | Ninety-six isolates of sunflower Sclerotinia sclerotiorum (Lib.) de Bary from Inner Mongolia (IM) in China, from Canada and the United Kingdom (UK) were sampled to investigate the
genetic diversity and structure using Sequence-Related Amplified Polymorphism. A total of 123 polymorphic bands were obtained,
ranging in size from 100 to 500 base pairs. The five populations of S. sclerotiorum isolated from the three countries showed various levels of genetic variability. The percentage of polymorphic loci varied
from 30.89% in the UK population to 97.56% in the Middle IM population. The values of Shannon index (i) varied from 0.1876 in the UK population to 0.5301 in the West IM population. The heterozygosity of the five geographic populations
obtained by estimating allele frequency varied from 12.91% in the UK population to 35.44% in the West IM population. The genetic
identity, as indicated by the Nei unbiased identity index, ranged from 0.9744 between populations from Canada and East IM
to 0.6477 between populations from West IM and UK. UPGMA cluster analysis using Nei’s genetic distance gave distances ranging
from 0.0259 to 0.4343. The rates of gene flow among five geographic populations ranged from 1.5406 between West IM and UK
populations to 18.4149 between West IM and Middle IM populations. The four populations from West IM, Middle IM, East IM and
Canada were clustered into one subgroup in which the isolates from West and Middle IM belonged to one population, whereas
those from East IM and Canada essentially were another population. The isolates from the UK formed a population that was significantly
distinct from other populations. |
| |
Keywords: | Sclerotinia sclerotiorum Sequence-related amplified polymorphism Genetic diversity Population structure |
本文献已被 SpringerLink 等数据库收录! |
|