中华乌塘鳢精子的生物学特性及其超低温保存

对中华乌塘鳢精子激活的生理生态学进行了研究，探讨了中华乌塘鳢精子超低温保存方法，中华乌塘鳢精子激活的适宜盐度为５－１５；其精子的激活不仅与激活溶溶盐度有关，而且与激活溶液的离子成份有关，激活溶液中Ｋ＾＋的存在一定程度上对精子的活动有抑帛和；中华乌塘鳢精子对ＰＨ的适应性强，在ＰＨ５．５－９．５范围内，激活率均在７０９％以上，尤以中性及弱酸性条件下的激活率最高，在ＰＨ６．０时精子活力最好。使用筛选出的

The biological characteristics of Bostrichthys sinensis spermatozoa and its cryopreservation

Effect of environmental factors on the activation of spermatozoa of Chinese ocellated sleeper, Bostrichthys sinensis ( Lac.p.de) , was studied. The optimum activat ing salinity for the spermatozoa was 5- 15. The activation of the spermatozoa concerned with not only osmosis or salinity, but also with composition of solution. The function of K+ was represented as a restraining factor to activation and movement of the spermatozoa in the present condition. The spermatozoa had a strong adaptability to the variation of pH, and the activating rates were more than 70% while pH of the activating solution varied from 5. 5 to 9. 5. The optimum pH of the activating solution was neutral or weak acidity for the spermatozoa, the swimming time of the spermatozoa was the longest when pH was 6. 0. The spermatozoa of Chinese ocellated sleeper were cryopreserved successfully by using a simple procedure . Sea water with salinity 40 or a solution containing 1. 5% NaCl , 1 . 5% KCl , 0. 25% CaCl2 and 0. 25% MgCl2, was practical extender for cryopreservation of Chinese ocellated sleeper spermatozoa. DMSO was an effective cryoprotectant, and its optimum concentration was 10% in this study. The activation rates of the pos-t thaw spermatozoa were almost as good as the control. s , namely fresh spermatozoa, and their fertilizat ion rates were over 80%, approaching the control. s.