上海临港滨海河道夏季周丛生物群落演替特征

于2021年6月26日-7月10日对上海浦东地区芦潮引河3段河流进行连续挂板采样，研究不同污染程度河流中周丛生物的分布特征及时间变化。共采集12次，获得36个样品，对18S rDNA V4可变区进行高通量测序。结果发现，主要类群为轮虫、绿藻、纤毛虫。不同污染程度河流周丛生物群落组成呈现显著性差异(p<0.01)，污染最严重的Y点样品优势属为Pentatrocha、Lagenidium、Dinovorax、Symbiodinium等，其特有属有Calycofera、Crucigeniella等；污染较为严重的H点样品的优势属为Ptygura、Oedogonium、Philodina、Physocypria等，特有属有Eccrinidus、Hyphochytrium等；无污染的G点样品优势属为Cyclotella、Prunus、Vorticella、Chlamydomonas等，特有属有Actinochloris、Adelina、Albugo等。生物群落与环境因子的趋势对应显示，TDS、盐度、pH和污染程度为影响周丛生物群落变化的显著性因素。不同生物对于环境因子的响应程度不同，优势属对于环境因子的选择性趋势更加明显。主要周丛生物类群（纤毛虫、轮虫、绿藻）在不同污染程度河流中群落丰度变化不同，调查周期内，在受到污染的G点和Y点周丛生物群落出现了绿藻代替纤毛虫的群落演替趋势。本研究为受污染水体周丛生物生态功能评估及环境评估提供基础数据，也为周丛生物（膜）作为一种环保绿色、造价低廉的污染物去除方法提供理论基础。

Succession of periphyton communities in the Shanghai Lingang coastal river in summer

We conducted a 14-day cycle investigation on the temporal variations of periphyton communities at three sampling sites from June to July 2021 in river within the Shanghai Pudong New Aera. A total of 36 samples were collected including three stations, and High Throughput Sequencing (V4 region of 18S rDNA) were employed. Results showed that rotifers, chlorophyta and ciliates were dominant as periphyton. The composition of periphyton communities in rivers with different pollution levels showed significant differences (p<0.01), and the dominant genus in the most polluted samples(Y) were Pentatrocha, Lagenidium, Dinovorax, Symbiodinium, etc., and endemic genus were Calycofera, Crucigeniella, etc.; The dominant genera of the more severely polluted samples(H) were Ptygura, Oedogonium, Philodina, Physocypria, etc., and the endemic genus were Eccrinidus, Hyphochytrium, etc.; the dominant genus of the non-polluting samples(G) were Cyclotella, Prunus, Vorticella, Chlamydomonas, etc., and the endemic genus were Actinochloris, Adelina, Albugo, etc. TDS, S, pH and pollution level were the significant environmental factors affecting the composition of periphyton communities. And the dominant genus showed more pronounced trend to environmental factors. The community abundance of the main periphyton taxa (ciliates, rotifers, chlorophyta) varied in rivers with different pollution levels, and the community succession trend of chlorophyta replacing ciliates was shown at G and H during the study time period. This study provides basic data for ecological function assessment and environmental assessment of periphyton in polluted water bodies, and also provides a theoretical basis for periphyton biomass (membrane) as an environmentally friendly, green and inexpensive pollutant removal method.