超声波辅助提取番木瓜提取液抗氧化活性研究

采用DPPH自由基清除率研究超声波辅助提取番木瓜乙醇提取液的抗氧化能力。主要研究提取温度、提取时间和提取功率对番木瓜乙醇提取液抗氧化活性的影响,并且以DPPH.清除率最大值为衡量标志,采用单因素和正交试验结合确定最佳提取条件,同时测定了番木瓜乙醇提取液中总多糖、总黄酮和总多酚的含量,研究了番木瓜乙醇提取液中总多糖、总黄酮和总多酚的含量和DPPH.清除率的相关性。通过正交试验研究得到的结论为:对以无水乙醇为溶剂番木瓜提取液,以DPPH.清除率最大为抗氧化活性衡量标准超声辅助提取最佳工艺条件为:提取时间30 min,提取温度40℃,超声功率为600W;3个因素对番木瓜乙醇提取液DPPH.清除率影响程度为:提取时间>提取温度>超声功率。番木瓜乙醇提取液的总多糖、总黄酮和总多酚含量与DPPH.清除率无可循相关性。

Studies on the Anti-oxidant Activity of Ultrasonic-assisted Extraction from Fruit of Carica Papaya

The DPPH radical scavenging activities was used as the main indicator of the antioxidant capacity of ultrasonic-assisted ethanol extracts from fruit of Carica papaya in different condition.Effects of extraction temperature,extraction time and extraction ultrasonic power on antioxidant activities of ethanol extracts from fruit of Carica papaya were studied.The maximum of clearance to DPPH was used as evaluating criteria.The best extracting condition was optimized by single factor and the orthogonal experiments.The content of total polysaccharide,total flavonoids and total polyphenols in these ethanol extracts were determined.The relationship among the content of total polysaccharide,total flavonoids,total polyphenols and the DPPH radical scavenging activities in these ethanol extracts was studied.Through orthogonal experiments,the optimal extraction conditions were determined: extraction time 30 min,extraction temperature 40℃,extraction ultrasonic power 400W.The influence degree of three factors on the extraction condition was the extraction time > the extraction temperature > the extraction ultrasonic power.No notable relationship among the content of total polysaccharide,total flavonoids,total polyphenols and the DPPH radical scavenging activities in these ethanol extracts was found.