| 桃果实中花青苷的提取、检测及应用 |
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| 引用本文： | 胡亚东,贾惠娟,孙崇德,陈青俊,徐昌杰,陈昆松. 桃果实中花青苷的提取、检测及应用[J]. 果树学报, 2004, 21(2): 167-169 |
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| 作者姓名： | 胡亚东 贾惠娟 孙崇德 陈青俊 徐昌杰 陈昆松 |
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| 作者单位： | 1. 浙江大学果实分子生理与生物技术实验室,农业部园艺植物生长发育与生物技术重点开放实验室,浙江,杭州,310029
2. 浙江省食品质量监督检验站,浙江,杭州,310009 |
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| 基金项目： | 国家自然科学基金(30170660)，浙江省自然科学基金重点(ZD0004)项目资助 |
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| 摘 要： | 采用0.05%盐酸-甲醇提取液4℃浸取组织24h,可以高效提取桃果实果皮中的花青苷,方法回收率可达94.37%;采用梯度洗脱HPLC法,可以对花青苷进行定性、定量分析,该体系检测限(S/N=3)为0.2μg/g,RSD(n=5)为2.94%,峰面积与浓度在0.0625～0.2500mg/mL范围内线性关系达到极显著水平(r=0.997)。方法简便易行,可用于桃果实等材料的花青苷水平变化分析。
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| 关 键 词： | 果实 花青苷 高效液相色谱 梯度洗脱 提取 检测 |
| 文章编号： | 1009-9980(2004)02-167-03 |
| 修稿时间： | 2003-05-10 |
A Method for Extracting and Determining Anthocyanin From Peach Fruit and Its Application |
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| Abstract: | Using peach fruit as material we established an effective extr action and analysis system.Anthocyanins could be efficiently extracted from pea ch epidermis with0.05%HCl-methanol solution for24h at4℃.Gradient elu tion was applied in HPLC analysis,and the recovery was found to be as high as9 4.37%,and the limit of determination(S/N=3)was0.2ìg/g.There was a significantly positive correlation between HPLC peak area and anthocyanin con centration ranging from0.0625to0.25mg/mL,with a correlative coeffic ient of0.997and a RSD of2.94%.The system was simple,feasible an d can be used to analyze anthocyanin in peach fruit. |
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| Keywords: | Fruit Anthocya nin HPLC Gradient elution Extraction Determination |
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