Two powdery mildew resistance mutations induced by ENU in Pisum sativum L. affect the locus er1 |
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Authors: | Gra?a Pereira and José Leit?o |
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Institution: | (1) BioFIG, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal;(2) INRB, L-INIA/ex-ENMP, Apartado 6, 7350-951 Elvas, Portugal; |
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Abstract: | The alkylating compounds and strong mutagens methylnitrosourea (MNU) and ethylnitrosourea (ENU) were used to widen the genetic
variability of pea (Pisum sativum L.) via experimental mutagenesis. Amongst multiple mutations of agronomical interest, for the first time two mutations conferring
broad-range resistance to powdery mildew (Erysiphe pisi Syd.) were induced in pea. Mutagenic treatments were carried out on seedlings using a technique that ensures very high mutagenic
efficiency. Two-hour exposure of cv. Solara seedlings to chemical mutagens resulted in almost non noticeable lethality and
sterility in the M1 generation and very high mutation rates: ~39% families with visible mutations in the M2 generation. The
influence of the cell cycle phase on the process of mutagenesis was studied in cv. Frilene using a previously developed technique
for synchronization of shoot apical meristem cells. The cell cycle phase at which cells were treated apparently did not influence
the lethality and sterility in the M1 generation, while the visible mutation rate, assessed in the M2 generation, showed a
clear cell cycle dependency. Seedlings treated at the G2 and M phases gave rise to progenies exhibiting the highest mutation
rate, over 50% of M2 families with visible mutations. The powdery mildew resistant (PMR) mutant S(er1mut1) was induced by treatment of cv. Solara seedlings for 2 h with ENU, while the PMR mutant F(er1mut2) was induced by treatment of cv. Frilene seedlings with the same chemical mutagen for 1 h during the G2 phase of the cell
cycle. The genetic analysis of the novel PMR mutant lines showed that both resistances are inherited as monogenic recessive
traits. The performed genetic complementation analyses revealed that both mutations affect the same locus—er1, which determines most of the natural sources of PMR in pea. A project aiming at the isolation of the powdery mildew resistance
mutated gene via map based cloning is currently under way. |
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