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Effect of titanium ascorbate on the lipoxygenase pathway of tomato and red pepper seedlings
Authors:C Sarmiento  H Daood  I Pais  P A Biacs
Institution:1. Central Food Research Institute (KéKI) , P. O. Box 393, Budapest, H‐1536, Hungary;2. Brassica and Oilseeds Research Department , John Innes Centre , Colney, Norwich, NR4 7UH, United Kingdom;3. Central Food Research Institute (KéKI) , P. O. Box 393, Budapest, H‐1536, Hungary;4. Department of Chemistry , University of Horticulture and Food Industry , Villány út, 37, Budapest, H‐1118, Hungary
Abstract:The effect of titanium applied as titanium ascorbate (Titavit®) on the lipoxygenase (LOX) pathway of tomato and red pepper seedlings was studied. Seeds were germinated in solutions containing titanium at 5 and 10 ppm. The uptake of titanium was significant and occurred at a similar amount for both tomato and red pepper. LOX and hydroperoxide decomposing (HPD) activities were determined in the crude extracts. The results showed that LOX activity in the extract of Titavit‐germinated seedlings was significantly higher than that of the control, whereas no effect was observed on the activity of HPD enzymes. It is suggested that activation of LOX by Titavit was due to a chemical interaction of titanium within the reaction mixture than to an induction of the biosyntheis of the enzyme.
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