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ACCUMULATION AND TRANSLOCATION OF SILICON IN RICE AND BEAN PLANTS USING THE 30SI STABLE ISOTOPE
Authors:J M T Carneiro  L A Oliveira  A L R M Rossete  C H Abreu Jr  J A Bendassolli
Institution:Center for Nuclear Energy in Agriculture , University of S?o Paulo , Piracicaba, Brazil
Abstract:The 30Si silicon isotope stable was used for assessing the accumulation and translocation of Si in rice and bean plants grown in labeled nutritive solution. The isotopic silicon composition in plant materials was determined by mass spectrometry (IRMS) using the method based on SiF4 formation. Considering the total-Si added into nutritive solutions, the quantity absorbed by plants was near to 51% for rice and 15% for bean plants. The accumulated amounts of Si per plant were about 150g in rice and 8.6g in bean. Approximately 70% of the total-Si accumulated was found in leaves. At presented experimental conditions, the results confirmed that once Si is accumulated in the old parts of rice and bean plant tissues it is not redistributed to new parts, even when Si is not supplied to plants from nutritive solution.
Keywords:silicon  stable isotopes  isotopic determination  labeled compounds  mass spectrometry  plants
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