| 二甲双胍对鸡巨噬细胞HD11天然免疫相关基因表达影响及其抗禽白血病病毒能力分析 |
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| 引用本文： | 豆春峰,王芳,胡序明,欧阳娟,陈世豪,贾崇信,薛松磊,徐慧,齐田羽,耿拓宇,崔恒宓.二甲双胍对鸡巨噬细胞HD11天然免疫相关基因表达影响及其抗禽白血病病毒能力分析[J].中国动物传染病学报,2019(3):22-26. |
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| 作者姓名： | 豆春峰 王芳 胡序明 欧阳娟 陈世豪 贾崇信 薛松磊 徐慧 齐田羽 耿拓宇 崔恒宓 |
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| 作者单位： | 扬州大学表观遗传学及表观基因组学研究所;扬州大学动物科学和技术学院;扬州大学兽医学院;扬州大学比较医学研究院;江苏高校动物重要疫病与人兽共患病防控协同创新中心;教育部农业与农产品安全国际合作联合实验室 |
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| 基金项目： | 国家自然科学基金重大研究计划(91540117);国家自然科学基金面上项目(81372237);国家自然科学基金青年基金项目(31602032);江苏省畜牧学优势学科项目;江苏省高等学校大学生创新创业训练计划项目(201611117037Z) |
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| 摘 要： | 本研究旨在探讨二甲双胍(metformin)处理对鸡巨噬细胞HD11天然免疫相关基因表达及禽白血病病毒(Avian leukosisvirus,ALV)复制水平的影响。用2mmol/L二甲双胍处理鸡巨噬细胞HD11,处理48h后收集细胞,荧光定量PCR(RT-qPCR)检测天然免疫基因和内源性反转录病毒的表达。另外,将J亚群禽白血病病毒(J subgroup Avian leukosis virus,ALV-J)(MOI=5)接种于细胞板中,病毒感染细胞24 h后进行二甲双胍处理, 48 h后收集HD11细胞和上清分别进行RT-qPCR检测和ELISA(enzyme linked immunosorbent assay)检测。结果表明,二甲双胍显著上调HD11细胞天然免疫相关基因TLR3(P<0.01)、IFIH1(P<0.01)、IRF7(P<0.01)、STAT1(P<0.05)、IFN-α(P<0.01)、IFN-β(P<0.01)表达水平,并激活了鸡内源性反转录病毒的表达,抑制ALV-J复制水平。综上,二甲双胍可激活鸡巨噬细胞的天然免疫反应,从而提高鸡抗禽白血病病毒的能力。本研究在体外实验中验证二甲双胍具有抑制ALV增殖的作用,对家禽业的疾病防控有一定的应用价值。
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| 关 键 词： | 二甲双胍 禽白血病毒 天然免疫 巨噬细胞 |
EFFECT OF METFORMIN ON THE EXPRESSION OF INNATE IMMUNERELATED GENES AND THE ABILITY OF ANTI-AVIAN LEUCOSIS VIRUS IN CHICKEN MACROPHAGES HD11 |
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| DOU Chun-feng,WANG Fang,HU Xu-ming,OU Yang-juan,CHEN Shi-hao,JIA Chong-xin,XUE Song-lei,XU Hui,QI Tian-yu,GENG Tuo-yu,CUI Heng-mi.EFFECT OF METFORMIN ON THE EXPRESSION OF INNATE IMMUNERELATED GENES AND THE ABILITY OF ANTI-AVIAN LEUCOSIS VIRUS IN CHICKEN MACROPHAGES HD11[J].Chinese Journal of Animal Infectious Diseases,2019(3):22-26. |
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| Authors: | DOU Chun-feng WANG Fang HU Xu-ming OU Yang-juan CHEN Shi-hao JIA Chong-xin XUE Song-lei XU Hui QI Tian-yu GENG Tuo-yu CUI Heng-mi |
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| Institution: | (Institute of Epigenetics and Epigenomics, Yangzhou University, Yangzhou, Jiangsu 225009, China;College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China;College of Veterinary, Yangzhou University, Yangzhou, Jiangsu 225009, China;Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China;Joint International Research Laboratory of Agricultural & Agri-Product Safety, The Ministry of Education of China, Yangzhou University,Yangzhou, Jiangsu 225009, China) |
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| Abstract: | The aim of the study was to explore the effect of metformin on the expression of the innate immune-related genes and avian leukosis virus replication levels in chicken macrophages HD11. The HD11 cells were treated with 2 mmol/L metformin. After 48 h,cells were collected and the expression of innate immune-related genes and endogenous retroviruses were measured by fluorescence quantitative PCR(RT-qPCR). In addition, HD11 cells were infected with J subgroup avian leukosis virus(ALV-J)(MOI = 5). The cells were treated with metformin for 24 hours and collected for RT-qPCR and supernatant was collected after 48 hours for ELISA testing. The results show that metformin significantly activated the expression of innate immune-related genes TLR3(P<0.01), IFIH1(P<0.01), IRF7(P<0.01), STAT1(P<0.05), IFN-α(P<0.01), IFN-β(P< 0.01) and endogenous retroviruses. The level of avian leukosis virus replication decreased significantly. Therefore, metformin activated the innate immune responses of the chicken macrophages and enhanced the antiviral ability. |
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| Keywords: | Metformin Avian leukosis virus innate immunity macrophage |
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