表油菜素内酯对镉胁迫下番茄幼苗生长及镉累积的影响

采用盆栽试验研究不同浓度(0、0.02、0.1、0.5、2.5 mg/L)的表油菜素内酯(EBR)对镉胁迫下番茄幼苗生长及镉累积的影响。结果表明,镉处理后番茄幼苗的生物量、叶绿素含量、抗氧化酶(POD和CAT)活性均下降,丙二醛(MDA)含量、相对电导率和渗透调节物质含量均升高;喷施一定浓度的EBR(0.5 mg/L)后,番茄幼苗的生物量、叶绿素含量、抗氧化酶(POD和CAT)活性及脯氨酸含量均显著增加,可溶性蛋白、MDA含量和相对电导率有所下降,番茄幼苗根系和地上部分的镉含量分别上升了50.0%和93.4%,镉转运系数最低,为0.45。综上,喷施EBR能够增强番茄幼苗对镉胁迫的耐受性,促进幼苗生长,降低镉的转运系数,其中,EBR浓度为0.5 mg/L时效果最好。

Effects of epibrassinolide on the growth and cadmium accumulation of tomato seedlings under cadmium stress

A pot experiment was conducted to study the effects of epibrassinolide(EBR)with different concentrations(0,0.02,0.1,0.5,2.5 mg/L)on the growth and cadmium accumulation of tomato seedlings under cadmium stress.The results showed that the biomass,chlorophyll content and antioxidant enzymes(POD and CAT)activities of tomato seedlings decreased,while the contents of malondialdehyde,relative conductivity and osmotic regulators increased after cadmium treatment.After spraying EBR(0.5 mg/L)at a certain concentration,the biomass,chlorophyll content,antioxidant enzyme activity(POD and CAT)and proline content of tomato seedlings increased significantly,while the soluble protein,malondialdehyde and relative conductivity decreased,cadmium content in roots and aboveground parts of tomato seedlings increased by 50.0%and 93.4%respectively,and the cadmium transfer coefficient was the lowest at 0.45.In conclusion,spraying EBR could enhance the tolerance of tomato seedlings to cadmium stress,promote the growth of tomato seedlings,and reduce cadmium transport coefficient.EBR concentration of 0.5 mg/L was the best.