Determination of ammonium,amides, and soluble carboxylates in plant tissues |
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| Authors: | Helene M Roberts H M Reisenauer R M Carlson |
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| Institution: | 1. Department of Land, Air and Water Resources , University of California , Davis, California, 95616;2. Department of Pomology , University of California , Davis, California, 95616 |
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| Abstract: | Methods for extraction and determination of ammonium, amides and soluble carboxylates in plant tissues are compared and discussed. The procedure recommended involves extraction of finely ground plant tissues with 0.2 M formic acid, determination of ammonium and amides in the extract by overnight alkaline hydrolysis and distillation in Conway microdiffusion dishes, measurement of extracted ammonium using Carlson's rapid diffusion‐conductivity instrument, and estimation of soluble organic acids by titration after separation and purification with ion exchange resins. The method described is reasonably rapid, and gives complete recovery and highly reproducible results. The final solution of organic acids is suitable for direct chromatographic analysis. |
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| Keywords: | extraction methods organic acids |
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