| 塞尼卡病毒与口蹄疫病毒双重RT-PCR鉴别检测方法的建立 |
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| 引用本文： | 刘涛,黄元,陈锦良,向华,肖少波,王晓虎,陈晶,向蓉,何继军,郑海学.塞尼卡病毒与口蹄疫病毒双重RT-PCR鉴别检测方法的建立[J].中国动物传染病学报,2019(3):16-21. |
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| 作者姓名： | 刘涛 黄元 陈锦良 向华 肖少波 王晓虎 陈晶 向蓉 何继军 郑海学 |
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| 作者单位： | 广东省农业科学院动物卫生研究所广东省兽医公共卫生公共实验室广东省畜禽疫病防治研究重点实验室农业部兽用药物与诊断技术广东科学观测实验站;华中农业大学动物科学技术学院、动物医学院;中国农业科学院兰州兽医研究所 |
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| 基金项目： | 国家重点研发计划项目(2016YFD0501500);国家自然科学基金-广东联合基金项目(U1501213);广东省农业厅项目(2016LM2150);广东省农业科学院学科团队建设项目(201631TD);清远市科技计划项目(2017A003) |
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| 摘 要： | 2014~2015年,美国、巴西等国爆发了一种症状类似于口蹄疫的水泡性疾病,病原被确定为塞尼卡病毒A(Senecavirus A,SVA)。为了鉴别和诊断塞尼卡病毒A和口蹄疫病毒(Foot-and-mouth disease virus,FMDV),本研究针对塞尼卡病毒的VP1基因和口蹄疫的5’UTR基因,合成特异性引物,优化了反应体系和扩增条件,建立了一种可同时检测塞尼卡病毒A和口蹄疫病毒的双重RT-PCR方法。特异性和敏感性试验结果表明,建立的双重RT-PCR检测方法特异性好,敏感性强,对塞尼卡病毒A和口蹄疫病毒最低检出量分别为104 copies/μL、103 copies/μL。本研究建立的方法对临床快速鉴别塞尼卡病毒A和口蹄疫病毒感染具有重要意义。
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| 关 键 词： | 塞尼卡病毒A 口蹄疫病毒 双重RT-PCR |
DEVELOPMENT OF DOUBLE RT-PCR ASSAY FOR DIFFERENTIATION OF SENECAVIRUS AND FOOT-AND-MOUTH DISEASE VIRUS |
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| Institution: | (Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, P.R.China, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Guangdong Open Laboratory of Veterinary Public Health, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640,China;College of Animal Science&Technology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070,China;Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730070, China) |
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| Abstract: | In 2014-2015, a vesicular disease similar to foot-and-mouth disease(FMD) broke out in the United States and Brazil. Later,this pathogen was determined as Senecavirus A(SVA). In order to differentiate Senecavirus and Foot-and-mouth disease virus(FMDV),the specific primers were designed according to Senecavirus VP1 gene and FMD 5’UTR gene sequences for development of a PCR assay.The results showed that the developed double RT-PCR method had high sensitivity and specificity. The minimum detectable level was 104 copies/μL for Senecavirus and 103 copies/μL for FMDV. The availability of this PCR method provided a tool for rapid detection of FMDV and SVA. |
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| Keywords: | Senecavirus A Foot-mouth-disease virus double RT-PCR |
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