Differential Responses of Anti-Oxidative Enzymes to Aluminum Stress in Tolerant and Sensitive Soybean Genotypes |
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| Authors: | Yan Zhen Lv Miao Jing Su Shao-Hua Liu Ya-Le Yin Si-Si Wang |
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| Affiliation: | 1. NJU-NFU Joint Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences , Nanjing University , Nanjing, China;2. Key Laboratory of Forest Genetics &3. Biotechnology (Nanjing Forestry University) , Ministry of Education , Nanjing, China;4. NJU-NFU Joint Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences , Nanjing University , Nanjing, China |
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| Abstract: | Seedlings of two soybean genotypes, BX10 [aluminum (Al)-tolerant] and BD2 (Al-sensitive), were treated with Al to evaluate the relative root growth (RRG), callose content, Al-sensitive zone, lipid peroxidation, and the anti-oxidative enzyme activities by histochemical and biochemical assays. Under Al toxicity, the RRG reduction of BD2 was more significant than that of BX10, while callose content displayed a contrary trend. The 2–5 mm zone of root apex was the main Al-sensitive zone for soybeans. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) were found to be higher in the two genotypes under Al toxicity than that of the controls. The SOD, POD, and CAT activities of BX10; however, were significantly lower than those of BD2. These results implied that producing low quantity of ROS may be one aspect of the Al-tolerant mechanism for soybeans, which in turn helps them adapt to Al stress. |
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| Keywords: | soybean Al toxicity oxidative stress anti-oxidative enzymes Al-sensitive zone |
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