EFFECTS OF CARBOHYDRATE SOURCE ON THE IN VITRO ASYMBIOTIC SEED GERMINATION OF THE TERRESTRIAL ORCHID HABENARIA MACROCERATITIS |
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| Authors: | Scott L. Stewart Michael E. Kane |
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| Affiliation: | 1. Plant Restoration, Conservation, and Propagation Biotechnology Program, Environmental Horticulture Department , University of Florida , Gainesville, Florida, USA;2. Horticulture and Agriculture Programs , Kankakee Community College , Kankakee, Illinois, USA;3. Plant Restoration, Conservation, and Propagation Biotechnology Program, Environmental Horticulture Department , University of Florida , Gainesville, Florida, USA |
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| Abstract: | The effects of the carbohydrates fructose, sucrose, and dextrose on the seed germination and protocorm development of the terrestrial orchid Habenaria macroceratitis were assessed in the presence or absence of banana powder. Malmgren Modified Terrestrial Orchid Medium was used as the basal medium. Each carbohydrate was screened at 50 mM with and without banana powder (15 g L?1). No significant differences in germination or protocorm development among all treatments were observed after seven weeks. There were no significant differences in advanced development among carbohydrate treatments without banana powder after 21 weeks. However, the presence of banana powder in the medium suppressed advanced protocorm development in the fructose, dextrose, and control treatments. These data support the wide suitability of simple exogenous carbohydrates in supporting the germination and protocorm development of H. macroceratitis, and suggest that a wide range of carbohydrates are suitable for the culture of often difficult-to-germinate terrestrial orchids. |
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| Keywords: | asymbiotic carbohydrate dextrose fructose Habenaria macroceratitis Orchidaceae seed germination sucrose terrestrial orchid |
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