| 藿香植物组织培养技术研究 |
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| 引用本文： | 李红,李永文,寇凤仙,周彦珍,温秀荣. 藿香植物组织培养技术研究[J]. 北方园艺, 2007, 0(9): 195-197 |
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| 作者姓名： | 李红 李永文 寇凤仙 周彦珍 温秀荣 |
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| 作者单位： | 保定职业技术学院,农林与生物工程系,河北,保定,071051 |
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| 摘 要： | 选用藿香健壮枝条和叶片作为试验材料,进行组培技术的研究.结果表明:藿香取材季节以夏季较好,适宜的外植体种类为茎段.诱导愈伤组织形成的激素组合为6-BA 1.0mg/L NAA 0.3 mg/L 2,4-D 0.3 mg/L,具有愈伤组织生长速度快并易于分化;不定芽分化激素组合为6-BA 0.5 mg/L NAA 0.1 mg/L,继代培养组合6-BA 0.1 mg/L NAA 0.5 mg/L B90.3mg/L,增殖在8倍以上;生根培养组合1/2MS NAA0.6 mg/L,一般20 d左右生根,平均根量4～5条,生根率达92%.
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| 关 键 词： | 藿香 植物组织培养 外植体 |
| 文章编号： | 1001-0009（2007）09-0195-03 |
| 收稿时间： | 2007-04-04 |
| 修稿时间： | 2007-04-04 |
Study on the Plant Tissue Culture Technology of Agastache rugasa (Fisch. et Meyer) O. Ktze |
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| LI Hong,LI Yong-wen,KOU Feng-xian,ZHOU Yan-zhen,WEN Xiu-rong. Study on the Plant Tissue Culture Technology of Agastache rugasa (Fisch. et Meyer) O. Ktze[J]. Northern Horticulture, 2007, 0(9): 195-197 |
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| Authors: | LI Hong LI Yong-wen KOU Feng-xian ZHOU Yan-zhen WEN Xiu-rong |
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| Affiliation: | Baoding Vocational and Technical College, Hebei 071051 ,China |
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| Abstract: | Selectedthe vigorous and healthy branch and leaf of Agastache rugasa(Fisch.et Meyer) O.Ktze as explants carried on the plant tissue culture technology research.The results showed that summer was the best season for plant tissue culture,plant hormone combination of 6-BA 1.0 mg/L NAA 0.3 mg/L 2,4-D 0.3 mg/L was good for callus induction,6-BA 0.5 mg/L NAA 0.1 mg/L was suitable for Adventitious bud formation,6-BA 0.1 mg/L NAA 0.5 mg/L B90.3mg/L was good for cultivation of young plant,the coefficient of reproduction was up to 8 times.NAA0.6 mg/L was best for rootage,rooting rate was 92%. |
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| Keywords: | Agastache rugasa(Fisch.et Meyer) O.Ktze Plant tissue culture Explant |
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