| 百脉根花对称性基因LjCYC3转化烟草的研究 |
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| 引用本文： | 张寒英,胡江琴,向太和,梁海曼,王利琳.百脉根花对称性基因LjCYC3转化烟草的研究[J].浙江大学学报(农业与生命科学版),2011,37(1). |
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| 作者姓名： | 张寒英 胡江琴 向太和 梁海曼 王利琳 |
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| 作者单位： | 1. 杭州师范大学,生命与环境科学学院,浙江,杭州,310036
2. 浙江大学,生命科学学院,浙江,杭州,310058 |
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| 摘 要： | 根据百脉根花对称性基因LjCYC3构建反义表达载体,经农杆菌介导正、反义LjCYC3基因转化烟草,以获得花型改变的转基因烟草植株,并根据表型选取正、反义各3株转基因植株和1株野生型植株进行Q-PCR分析,观察外源基因在远源植物中的作用和影响.PCR分析结果表明:转基因植株中正义基因和反义基因的转化率分别为62.5%和71%.表型观察显示:30%的正义转基因植株主要表现为花瓣和雄蕊数目减少;而40%的反义转基因植株表现为2朵花有一定程度的融合,花冠萼片有褪色现象,雄蕊端头有花瓣状结构,雌蕊2枚且与雄蕊有合生现象.Q-PCR分析结果表明:在正义转基因再生植株中,外源基因均高效表达,随着表达量的增加,花型变化更加明显;而在反义转基因再生植株中,外源基因也有表达,随着表达量的降低,花型变化更加明显;与正义基因表达量相比,反义基因表达量要小约150倍.本研究结果为下阶段用该基因转化花卉植物以改变花型、改良花卉品种打下了实验基础.
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| 关 键 词： | 百脉根 LjCYC3 基因 正、反义表达载体 烟草 |
Study on transformation of tobacco plant with flower symmetry gene LjCYC3 in Lotus japonicus |
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| ZHANG Han-ying,HU Jiang-qin,XIANG Tai-he,LIANG Hai-man,WANG Li-lin.Study on transformation of tobacco plant with flower symmetry gene LjCYC3 in Lotus japonicus[J].Journal of Zhejiang University(Agriculture & Life Sciences),2011,37(1). |
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| Authors: | ZHANG Han-ying HU Jiang-qin XIANG Tai-he LIANG Hai-man WANG Li-lin |
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| Institution: | ZHANG Han-ying1,HU Jiang-qin1,XIANG Tai-he1,LIANG Hai-man2,WANG Li-lin1 (1.College of Life and Environment Sciences,Hangzhou Normal University,Hangzhou 310036,China,2.College of Life Sciences,Zhejiang University,Hangzhou 310058,China) |
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| Abstract: | According to LjCYC3 gene controlling flower symmetry in Lotus japonicus,the antisense gene expression vector was constructed,and by using Agrobacterium-mediated transformation of tabacoo with justice-antisense LjCYC3 gene,flower patterns changed in transgenic tobacco plants were obtained,and then three transgenic plants and a wild-type plant were selected for Q-PCR analysis according to phenotype.PCR analysis showed that transformation rates of justice gene and antisense gene were 62.5% and 71% in transgeni... |
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| Keywords: | Lotus japonicus LjCYC3 gene justice-antisense expression vector tobacco |
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