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| 水稻条斑病菌avrBs3/pthA家族基因敲除体系的建立 | |
| 引用本文： | 曹燕飞,邹丽芳,赵文祥,纪志远,邹华松,陈功友.水稻条斑病菌avrBs3/pthA家族基因敲除体系的建立[J].浙江大学学报(农业与生命科学版),2011,37(1). |
| 作者姓名： | 曹燕飞 邹丽芳 赵文祥 纪志远 邹华松 陈功友 |
| 作者单位： | 1. 南京农业大学,植物保护学院,农业部病虫监测与治理重点开放实验室,江苏,南京,210095 2. 上海交通大学,农业与生物学院,上海,200240 3. 南京农业大学,植物保护学院,农业部病虫监测与治理重点开放实验室,江苏,南京,210095;上海交通大学,农业与生物学院,上海,200240 |
| 基金项目： | 农业部公益行业专项资助项目，转基因重大专项资助项目 |
| 摘 要： | 水稻细茵性条斑病菌存在至少20个avrBs3/pthA家族成员,但其在水稻上的毒性或无毒性贡献并不清楚.本文依据avrBs3/pthA结构上的保守性,利用同源重组策略,借助自杀性载体pKMS1介导,对水稻条斑病菌中的avrBs3/pthA家族基因进行敲除.结果发现:同源交换可通过基因内和基因间重组实现avrBs3/pthA家族的基因敲除;PCR和Southern杂交结果显示,水稻条斑病菌中有5个avrB5s3/pthA家族基因被成功敲除,表明水稻条斑病菌avrBs3/pthA家族基因敲除体系构建成功.这为逐一评价avrBs3/pthA 家族基因的毒性或/和无毒性功能奠定了基础. |
| 关 键 词： | 水稻条斑病菌 avrBs3/pthA基因 基因敲除 遗传重组 |
Establishment of knockout mutagenesis in avrBs3/pthA family genes of Xanthomonas oryzae pv. Oryzicola |
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| CAO Yan-fei,ZOU Li-fang,ZHAO Wen-xiang,JI Zhi-yuan,ZOU Hua-song,CHEN Gong-you.Establishment of knockout mutagenesis in avrBs3/pthA family genes of Xanthomonas oryzae pv. Oryzicola[J].Journal of Zhejiang University(Agriculture & Life Sciences),2011,37(1). | |
| Authors: | CAO Yan-fei ZOU Li-fang ZHAO Wen-xiang JI Zhi-yuan ZOU Hua-song CHEN Gong-you |
| Institution: | CAO Yan-fei1,ZOU Li-fang2,ZHAO Wen-xiang1,JI Zhi-yuan1,ZOU Hua-song2,CHEN Gong-you1,2(1.Key Laboratory for Monitoring and Management of Plant Diseases and Insects,Ministry of Agriculture,College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China,2.College of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
| Abstract: | The contribution of at least twenty highly conserved avrBs3/pthA family genes in Xanthomonas oryzae pv.oryzicola(Xoc) to virulence or avirulence in rice is still rudimentary.Based on the conserved structure of the avrBs3/pthA family,a marker-exchange mutagenesis strategy in association with mediation by a suicide vector pKMS1 was used to knock out the avrBs3/pthA family genes of Xoc.The results showed that total five avrBs3/pthA genes were gradually knocked out based on the conserved structure of the family... |
| Keywords: | Xanthomonas oryzae pv oryzicola avrBs3/pthA family knockout mutagenesis recombination |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |