| 马传染性贫血病毒驴白细胞弱毒疫苗株致弱过程中不同代次病毒LTR的进化分析 |
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| 引用本文： | 王雪峰,杨彬,韩秀娥,林跃智,姜成刚,吕晓玲,赵利平,周建华,王凤龙. 马传染性贫血病毒驴白细胞弱毒疫苗株致弱过程中不同代次病毒LTR的进化分析[J]. 中国预防兽医学报, 2010, 32(12). DOI: 10.3969/j.issn.1008-0589.2010.12.01 |
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| 作者姓名： | 王雪峰 杨彬 韩秀娥 林跃智 姜成刚 吕晓玲 赵利平 周建华 王凤龙 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院;中国农业科学院哈尔滨兽医研究所兽医生物重点实验室/大动物病研究室;东北农业大学黑龙江省乳品工业技术开发中心; |
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| 基金项目： | 国家自然科学基金，国家十一五重大传染病专项 |
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| 摘 要： | 为揭示马传染性贫血病毒(EIAV)弱毒疫苗的减毒机理,本研究对EIAV弱毒疫苗株在体外驴白细胞传代过程中不同代次毒株的长末端重复序列(LTR)进行扩增和分析。结果显示:随着病毒在体外传代次数的增加,各病毒株遗传多样性逐渐增加,并与致弱前亲本株EIAVDV117的遗传距离逐渐增大;EIAV在体外传代过程中LTR的变异主要集中在U3区和R区的转录起始位点,但随着传代次数的增加,在负调节区丢失了GATA结合位点,并在增强子区出现了E-box基序。此外,传代初期低代次病毒株与后期的高代次弱毒株在负调节区的AP-1结合位点和转录起始位点以及TAR的起始位点存在明显差异。
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| 关 键 词： | 马传染性贫血病毒 长末端重复序列 进化分析 |
Genetic evolution of equine infectious anemia virus LTR region during attenuation in vitro |
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| WANG Xue-feng,YANG Bin,HAN Xiu-e,LIN Yue-zhi,JIANG Cheng-gang,LV Xiao-ling,ZHAO Li-ping,ZHOU Jian-hua,WANG Feng-long. Genetic evolution of equine infectious anemia virus LTR region during attenuation in vitro[J]. Chinese Journal of Preventive Veterinary Medicine, 2010, 32(12). DOI: 10.3969/j.issn.1008-0589.2010.12.01 |
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| Authors: | WANG Xue-feng YANG Bin HAN Xiu-e LIN Yue-zhi JIANG Cheng-gang LV Xiao-ling ZHAO Li-ping ZHOU Jian-hua WANG Feng-long |
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| Affiliation: | WANG Xue-feng1,2,YANG Bin1,HAN Xiu-e2,3,LIN Yue-zhi2,JIANG Cheng-gang2,LV Xiao-ling2,ZHAO Li-ping2,ZHOU Jian-hua2*,WANG Feng-long1*(1.College of Animal Science and Medicine,Inner Mongolia Agricultural University,Huhhot 010018,China,2.Division of Livestock Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,3.Heilongjiang Dairy Industry Technical Development Center,Northeast Agricultural U... |
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| Abstract: | Long terminal repeat(LTR) is an important region for equine infectious anemia virus(EIAV) genome in determining virulence and cell tropism.In this study,we examined the LTR populations in EIAV quasispecies of representative vaccine strains during its attenuation process in donkey monocyte-derived macrophages(MDMs) in vitro.The results demonstrated that the complexity and sequence diversity of LTRs in these quasispecies increased over passages in MDMs.The genetic distances of these MDMs-adapted EIAV strains ... |
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| Keywords: | equine infectious anemia virus long terminal repeat evolution analysis |
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